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Literature summary for 1.1.3.12 extracted from
- Synthesis of 4-pyridoxolactone from pyridoxine using a combination of transformed Escherichia coli cells (2008), J. Biosci. Bioeng., 106, 460-465.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | development of a simple and efficient synthesis for 4-pyridoxolactone starting with pyridoxine and using a whole-cell biotransformation by two transformed Escherichia coli cell type expressing the pyridoxine oxidase and the catalase, and chaperonin, while the second set expressed pyridoxal 4-dehydrogenase, overview. Pyridoxine is first oxidized to pyridoxal, which is then dehydrogenated to 4-pyridoxolactone by pyridoxine 4-oxidase and pyridoxal 4-dehydrogenase, respectively | Microbacterium luteolum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli strains JM109 and BL21(DE3), co-expression with catalase, chaperonin, and pyridoxal 4-dehydrogenase for construction of a biotransformation system catalyzing the formation of 4-pyridoxolactone from pyridoxine in whole cells, overview | Microbacterium luteolum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxine + O2 | Microbacterium luteolum | - |
pyridoxal + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Microbacterium luteolum | Q9AJD6 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
recombinant enzyme in Escherichia coli cells | Microbacterium luteolum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxine + O2 | - |
Microbacterium luteolum | pyridoxal + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNOX | - |
Microbacterium luteolum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Microbacterium luteolum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Microbacterium luteolum |
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Release 2023.1 (January 2023)
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