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Literature summary for 1.1.3.10 extracted from

  • Hassan, N.; Tan, T.C.; Spadiut, O.; Pisanelli, I.; Fusco, L.; Haltrich, D.; Peterbauer, C.K.; Divne, C.
    Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly (2013), FEBS Open Bio, 3, 496-504.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star DE3 cells Phanerodontia chrysosporium

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 0.1 M MnCl2, and 30% (w/v) polyethylene glycol 400 Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Phanerodontia chrysosporium
-
 2-dehydro-D-glucose + H2O2
-
 ?
D-glucose + O2 Phanerodontia chrysosporium K-3
-
 2-dehydro-D-glucose + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q6QWR1
-
-
Phanerodontia chrysosporium K-3 Q6QWR1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography and Superdex 200 gel filtration Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-3-fluoro-beta-D-glucose + H2O2 slow substrate Phanerodontia chrysosporium ?
-
 ?
3-deoxy-3-fluoro-beta-D-glucose + H2O2 slow substrate Phanerodontia chrysosporium K-3 ?
-
 ?
D-glucose + O2
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + H2O2
-
 ?
D-glucose + O2
-
 Phanerodontia chrysosporium K-3 2-dehydro-D-glucose + H2O2
-
 ?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
P2O
-
 Phanerodontia chrysosporium
pyranose 2-Oxidase
-
 Phanerodontia chrysosporium