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Literature summary for 1.1.3.10 extracted from

  • Pitsawong, W.; Sucharitakul, J.; Prongjit, M.; Tan, T.C.; Spadiut, O.; Haltrich, D.; Divne, C.; Chaiyen, P.
    A conserved active-site threonine is important for both sugar and flavin oxidations of pyranose 2-oxidase (2010), J. Biol. Chem., 285, 9697-9705.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
T169A the mutant shows severely decreased catalytic efficiency for D-glucose and D-galactose compared to the wild type enzyme Trametes ochracea
T169G the mutant shows decreased catalytic efficiency for D-glucose and D-galactose compared to the wild type enzyme Trametes ochracea
T169N the mutant shows decreased catalytic efficiency for D-glucose and increased catalytic efficiency for D-galactose compared to the wild type enzyme Trametes ochracea
T169S the mutant shows increased kcat and Km values for D-glucose and D-galactose compared to the wild type enzyme Trametes ochracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
O2 wild type enzyme, using D-galactose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.07
-
O2 mutant enzyme T169S, using D-galactose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.09
-
O2 wild type enzyme, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.2
-
O2 mutant enzyme T169A, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.4
-
O2 mutant enzyme T169A, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.9
-
D-glucose mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.99
-
O2 mutant enzyme T169S, using D-glucose as cosubstrate ,in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
1.13
-
D-glucose wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
1.32
-
O2 mutant enzyme T169N, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
1.7
-
D-glucose mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
3.1
-
D-galactose mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
3.7
-
O2 mutant enzyme T169G, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
4.5
-
O2 mutant enzyme T169G, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
4.6
-
D-glucose mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
4.8
-
O2 mutant enzyme T169N, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
15
-
D-galactose wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
16
-
D-galactose mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
16.2
-
D-galactose mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
30
-
D-glucose mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
50
-
D-galactose mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea

Organism

Organism UniProt Comment Textmining
Trametes ochracea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + O2
-
Trametes ochracea 2-dehydro-D-galactose + H2O2
-
?
D-glucose + O2
-
Trametes ochracea 2-dehydro-D-glucose + H2O2
-
?

Synonyms

Synonyms Comment Organism
P2O
-
Trametes ochracea
pyranose 2-Oxidase
-
Trametes ochracea
pyranose:oxygen 2-oxidoreductase
-
Trametes ochracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
D-glucose mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.7
-
D-galactose mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
1.2
-
D-galactose wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
5.6
-
D-glucose mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
9.7
-
D-glucose wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
13.7
-
D-galactose mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trametes ochracea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
D-glucose mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.005
-
D-galactose mutant enzyme T169A, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.07
-
O2 mutant enzyme T169A, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.08
-
D-galactose wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.15
-
D-galactose mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.2
-
O2 mutant enzyme T169G, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.3
-
D-galactose mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.4
-
O2 mutant enzyme T169A, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.4
-
O2 mutant enzyme T169G, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.5
-
D-galactose mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
0.8
-
D-glucose mutant enzyme T169G, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
0.99
-
O2 mutant enzyme T169S, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
1.2
-
D-glucose mutant enzyme T169N, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
3.4
-
O2 mutant enzyme T169N, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
4.8
-
O2 mutant enzyme T169N, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
8.1
-
D-glucose mutant enzyme T169S, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
8.6
-
D-glucose wild type enzyme, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea
32
-
O2 mutant enzyme T169S, using D-galactose as cosubstrate,in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
44.2
-
O2 wild type enzyme, using D-galactose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 25°C Trametes ochracea
110
-
O2 wild type enzyme, using D-glucose as cosubstrate, in 50 mM sodium phosphate (pH 7.0), at 4°C Trametes ochracea