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Literature summary for 1.1.3.10 extracted from

  • Takakura, Y.; Kuwata, S.
    Purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete,Tricholoma matsutake (2003), Biosci. Biotechnol. Biochem., 67, 2598-2607.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Tricholoma matsutake

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.28
-
D-glucose pH 7.0, 37°C Tricholoma matsutake
10.7
-
1,5-anhydro-D-glucitol pH 7.0, 37°C Tricholoma matsutake
26.4
-
L-sorbose pH 7.0, 37°C Tricholoma matsutake
45
-
D-galactose pH 7.0, 37°C Tricholoma matsutake
45.8
-
D-xylose pH 7.0, 37°C Tricholoma matsutake
174
-
D-arabinose pH 7.0, 37°C Tricholoma matsutake
192
-
trehalose pH 7.0, 37°C Tricholoma matsutake
295
-
D-mannose pH 7.0, 37°C Tricholoma matsutake
329
-
D-maltose pH 7.0, 37°C Tricholoma matsutake

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
4 * 62000, SDS-PAGE Tricholoma matsutake
250000
-
gel filtration Tricholoma matsutake

Organism

Organism UniProt Comment Textmining
Tricholoma matsutake
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Tricholoma matsutake

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Tricholoma matsutake
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.8
-
-
Tricholoma matsutake

Storage Stability

Storage Stability Organism
-20°C, 0.1 M Tris-HCl, pH 7.0, enzyme retains 78% of the initial activity after 6 months in absence of glycerol Tricholoma matsutake
4°C, pH 7.0, enzyme retains more than 90% of the activity Tricholoma matsutake

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-glucitol + O2 18.4% of the activity with D-glucose Tricholoma matsutake ?
-
?
D-arabinose + O2 1.87% of the activity with D-glucose Tricholoma matsutake ?
-
?
D-galactose + O2 5.41% of the activity with D-glucose Tricholoma matsutake ?
-
?
D-glucose + O2
-
Tricholoma matsutake D-glucosone + H2O2
-
?
D-maltose + O2 15.1% of the activity with D-glucose Tricholoma matsutake ?
-
?
D-mannose + O2 6.02% of the activity with D-glucose Tricholoma matsutake ?
-
?
D-xylose + O2 13.4% of the activity with D-glucose Tricholoma matsutake ?
-
?
L-sorbose + O2 17.9% of the activity with D-glucose Tricholoma matsutake ?
-
?
trehalose + O2 14.3% of the activity with D-glucose Tricholoma matsutake ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 62000, SDS-PAGE Tricholoma matsutake

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Tricholoma matsutake

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 65% of maximal activity, 70°C: about 35% of maximal activity Tricholoma matsutake

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
30 min, stable Tricholoma matsutake
50
-
30 min, about 5% loss of activity Tricholoma matsutake
55
-
30 min, about 40% loss of activity Tricholoma matsutake
60
-
30 min, about 45% loss of activity Tricholoma matsutake
65
-
30 min, about 60% loss of activity Tricholoma matsutake
70
-
30 min, about 65% loss of activity Tricholoma matsutake

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.79
-
D-arabinose pH 7.0, 37°C Tricholoma matsutake
22.5
-
D-galactose pH 7.0, 37°C Tricholoma matsutake
25.1
-
D-mannose pH 7.0, 37°C Tricholoma matsutake
55.8
-
D-xylose pH 7.0, 37°C Tricholoma matsutake
59.6
-
trehalose pH 7.0, 37°C Tricholoma matsutake
62.9
-
D-maltose pH 7.0, 37°C Tricholoma matsutake
74.6
-
L-sorbose pH 7.0, 37°C Tricholoma matsutake
76.7
-
1,5-anhydro-D-glucitol pH 7.0, 37°C Tricholoma matsutake
111
-
D-glucose pH 7.0, 37°C Tricholoma matsutake

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Tricholoma matsutake

pH Range

pH Minimum pH Maximum Comment Organism
5.5 11 pH 5.5: about 40% of maximal activity, pH 11: about 40% of maximal activity Tricholoma matsutake

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
37°C, 30 min, 60% loss of activity Tricholoma matsutake
5.5
-
37°C, 30 min, about 15% loss of activity Tricholoma matsutake
7 11 37°C, 30 min, stable Tricholoma matsutake