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Literature summary for 1.1.3.10 extracted from

  • Leitner, C.; Volc, J.; Haltrich, D.
    Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor (2001), Appl. Environ. Microbiol., 67, 3636-3644.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
2,6-dichlorophenolindophenol pH 6.5, substrate: D-glucose Trametes ochracea
0.065
-
2,6-dichlorophenolindophenol at pH 6.5 and 30°C Trametes ochracea
0.07
-
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical pH 6.5, substrate: D-glucose Trametes ochracea
0.088
-
tetrachloro-1,4-benzoquinone pH 6.5, substrate: D-glucose Trametes ochracea
0.09
-
oxygen pH 6.5, substrate: D-glucose Trametes ochracea
0.09
-
tetrabromo-1,4-benzoquinone pH 6.5, substrate: D-glucose Trametes ochracea
0.09
-
tetrabromo-1,4-benzoquinone at pH 6.5 and 30°C Trametes ochracea
0.094
-
2,6-dichlorophenolindophenol pH 4.5, substrate: D-glucose Trametes ochracea
0.094
-
2,6-dichlorophenolindophenol at pH 4.5 and 30°C Trametes ochracea
0.22
-
tetrafluoro-1,4-benzoquinone pH 6.5, substrate: D-glucose Trametes ochracea
0.3
-
1,4-benzoquinone pH 4.5, substrate: D-glucose Trametes ochracea
0.31
-
1,4-benzoquinone pH 6.5, substrate: D-glucose Trametes ochracea
0.35
-
methyl-1,4-benzoquinone pH 4.5, substrate: D-glucose Trametes ochracea
0.35
-
methyl-1,4-benzoquinone at pH 4.5 and 30°C Trametes ochracea
0.4
-
methyl-1,4-benzoquinone pH 6.5, substrate: D-glucose Trametes ochracea
0.4
-
methyl-1,4-benzoquinone at pH 6.5 and 30°C Trametes ochracea
0.74
-
D-glucose
-
Trametes ochracea
0.83
-
2,6-dimethyl-1,4-benzoquinone pH 4.5, substrate: D-glucose Trametes ochracea
2.1
-
2,6-dimethyl-1,4-benzoquinone pH 6.5, substrate: D-glucose Trametes ochracea
3.9
-
5-thioglucose
-
Trametes ochracea
9.2
-
D-galactose
-
Trametes ochracea
30
-
D-xylose
-
Trametes ochracea
36
-
D-allose
-
Trametes ochracea
38
-
L-sorbose
-
Trametes ochracea
38
-
D-gluconolactone
-
Trametes ochracea
62
-
gentibiose
-
Trametes ochracea
97
-
L-arabinose
-
Trametes ochracea
110
-
D-mannoheptose
-
Trametes ochracea

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
4 * 65000, SDS-PAGE, corroborated by electron microscopy studies Trametes ochracea
262000
-
gel filtration Trametes ochracea
270000
-
equilibrium ultracentrifugation Trametes ochracea
278000
-
electron microscopy Trametes ochracea

Organism

Organism UniProt Comment Textmining
Trametes ochracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, hydrophobic interaction, ion-exchange chromatography, and gel filtration Trametes ochracea

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-sorbitol + O2
-
Trametes ochracea ?
-
?
1,6-anhydro-D-glucose + O2
-
Trametes ochracea ?
-
?
2,6-dimethyl-1,4-benzoquinone + O2
-
Trametes ochracea ? + H2O2
-
?
3-deoxy-D-glucose + O2
-
Trametes ochracea 2-keto-3-deoxy-D-glucose + H2O2
-
?
5-thioglucose + O2 24% relative activity to D-glucose Trametes ochracea 2-keto-5-thioglucose + H2O2
-
?
6-deoxy-D-glucose + O2
-
Trametes ochracea 2-keto-6-deoxy-D-glucose + H2O2
-
?
allose + O2 38% relative activity to D-glucose Trametes ochracea ?
-
?
D-fucose + O2
-
Trametes ochracea ?
-
?
D-galactose + O2 5.7% relative activity to D-glucose Trametes ochracea 2-dehydro-D-galactose + H2O2
-
?
D-gluconolactone + O2 64% relative activity to D-glucose Trametes ochracea ? + H2O2
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Trametes ochracea 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
r
D-glucose + methyl-1,4-benzoquinone
-
Trametes ochracea 2-dehydro-D-glucose + methylhydroquinone
-
r
D-glucose + O2 100% relative activity Trametes ochracea D-glucosone + H2O2
-
?
D-glucose + O2 D-glucose is the preferred substrate Trametes ochracea D-glucosone + H2O2
-
?
D-glucose + tetrabromo-1,4-benzoquinone
-
Trametes ochracea 2-dehydro-D-glucose + tetrabromohydroquinone
-
r
D-mannoheptose + O2 8.2% relative activity to D-glucose Trametes ochracea ?
-
?
D-xylose + O2 56% relative activity to D-glucose Trametes ochracea D-xylosone + H2O2
-
?
gentibiose + O2 23% relative activity to D-glucose Trametes ochracea 2-keto-D-gentibiose + H2O2
-
?
L-arabinose + O2 1.5% relative activity to D-glucose Trametes ochracea ?
-
?
L-idose + O2
-
Trametes ochracea ?
-
?
L-sorbose + O2 99% relative activity to D-glucose Trametes ochracea 5-dehydro-D-fructose + H2O2
-
?
tetrafluoro-1,4-benzoquinone + O2
-
Trametes ochracea ? + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 65000, SDS-PAGE, corroborated by electron microscopy studies Trametes ochracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
electron acceptor: 2,6-dimethyl-1,4-benzoquinone Trametes ochracea
4.5
-
electron acceptor: 2,6-dichloroindophenol Trametes ochracea
5.5 6.5 electron acceptor: oxygen or 1,4-benzoquinone Trametes ochracea
6 8 electron acceptor: tetrachloro-1,4-benzoquinone Trametes ochracea

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
half-life: 13 days Trametes ochracea
3.5
-
half-life: 52 days Trametes ochracea
4
-
half-life: 130 days Trametes ochracea
4.5
-
half-life: 350 days Trametes ochracea
5 7 extremely stable, half-life more than 500 days Trametes ochracea

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Trametes ochracea