Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | completely inhibits at 10 mM | Phanerodontia chrysosporium | |
ammonium sulfate | slightly inhibits | Phanerodontia chrysosporium | |
Cu2+ | 50% inhibition at 10 mM | Phanerodontia chrysosporium | |
Hg2+ | completely inhibits at 10 mM | Phanerodontia chrysosporium | |
Lead acetate | 50% inhibition at 10 mM | Phanerodontia chrysosporium | |
Mn2+ | 50% inhibition at 10 mM | Phanerodontia chrysosporium | |
Zn2+ | completely inhibits at 10 mM | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0832 | - |
oxygen | - |
Phanerodontia chrysosporium | |
0.82 | - |
D-glucose | oxygen concentration 0.25 mM | Phanerodontia chrysosporium | |
0.97 | - |
beta-D-glucose | oxygen concentration 1.07 mM | Phanerodontia chrysosporium | |
1.09 | - |
D-glucose | oxygen concentration 1.07 mM | Phanerodontia chrysosporium | |
1.43 | - |
D-glucose | at pH 8 | Phanerodontia chrysosporium | |
1.53 | - |
alpha-D-glucose | oxygen concentration 1.07 mM | Phanerodontia chrysosporium | |
25 | - |
D-xylose | oxygen concentration 1.07 mM | Phanerodontia chrysosporium | |
55 | - |
2-deoxy-D-glucose | oxygen concentration 1.07 mM | Phanerodontia chrysosporium | |
108 | - |
L-sorbose | oxygen concentration 1.07 mM | Phanerodontia chrysosporium | |
240 | - |
D-glucose | at pH 4.5 | Phanerodontia chrysosporium | |
257 | - |
maltose | oxygen concentration 1.07 mM | Phanerodontia chrysosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
4 * 65000, SDS-PAGE | Phanerodontia chrysosporium |
250000 | - |
PAGE under non-dissociating conditions | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using liquid chromatography on phenyl Sepharose, Mono Q twice and phenyl Superose | Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
- |
Phanerodontia chrysosporium |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl, pH 8, 30% glycerol, more than 1 year | Phanerodontia chrysosporium |
4°C, pH 4-11, retains more than 85% of the residual activity, 1 week | Phanerodontia chrysosporium |
repeated freezing at -20°C and thawing results in a rapid and irreversible loss of activity | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-beta-aurothioglucose + O2 | 91% relative activity to D-glucose | Phanerodontia chrysosporium | ? | - |
? | |
2-deoxy-D-glucose + O2 | 25% relative activity to D-glucose | Phanerodontia chrysosporium | 2-deoxy-3-keto-D-glucose + H2O2 | - |
? | |
3-O-methyl-D-glucose + O2 | 8.6% relative activity to D-glucose | Phanerodontia chrysosporium | 2-keto-3-O-methyl-D-glucose + H2O2 | - |
? | |
5-thioglucose + O2 | 30% relative activity to D-glucose | Phanerodontia chrysosporium | 2-keto-5-thioglucose + H2O2 | - |
? | |
6-deoxy-D-glucose + O2 | 76% relative activity to D-glucose | Phanerodontia chrysosporium | 2-keto-6-deoxy-D-glucose + H2O2 | - |
? | |
alpha-D-glucose + O2 | - |
Phanerodontia chrysosporium | 2-dehydro-D-glucose + H2O2 | - |
? | |
beta-D-glucose + O2 | - |
Phanerodontia chrysosporium | beta-2-dehydro-D-glucose + H2O2 | - |
r | |
D-fructose + O2 | 6.5% relative activity to D-glucose | Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
D-galactose + O2 | 4% relative activity to D-glucose | Phanerodontia chrysosporium | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-gluconolactone + O2 | 44% relative activity to D-glucose | Phanerodontia chrysosporium | ? + H2O2 | - |
? | |
D-glucose + O2 | D-glucose is the preferred substrate | Phanerodontia chrysosporium | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | alpha-D-glucose has a 104% relative activity compared to D-glucose, beta-D-glucose has a 80% relative activity compared to D-glucose | Phanerodontia chrysosporium | D-glucosone + H2O2 | - |
? | |
D-xylose + O2 | 30% relative activity to D-glucose | Phanerodontia chrysosporium | D-xylosone + H2O2 | - |
? | |
L-sorbose + O2 | 68% relative activity to D-glucose | Phanerodontia chrysosporium | 5-dehydro-D-fructose + H2O2 | - |
? | |
maltose + O2 | 26% relative activity to D-glucose | Phanerodontia chrysosporium | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 65000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
in 100 mM Tris, pH 8 | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Phanerodontia chrysosporium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | 50 mM acetate, the enzyme is relatively stable | Phanerodontia chrysosporium |
6 | - |
2-(N-morpholino)ethane sulfonic acid, the enzyme is relatively stable | Phanerodontia chrysosporium |
6 | 7 | bisTris, the enzyme is relatively stable | Phanerodontia chrysosporium |
8 | - |
optimal stability, 50 mM Tris-HCl, triethanolamine and imidazole, more than 95% of the residual activity remains after 36 days | Phanerodontia chrysosporium |
8 | - |
4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid, the enzyme is relatively stable | Phanerodontia chrysosporium |