Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | no significant effect | Phlebiopsis gigantea | |
2-mercaptoethanol | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
Ag+ | completely inhibits at 1 mM | Phlebiopsis gigantea | |
alpha,alpha'-dipyridyl | no significant effect | Phlebiopsis gigantea | |
ascorbic acid | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
Dithionite | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
dithiothreitol | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea | |
Fe2+ | completely inhibits at 1 mM | Phlebiopsis gigantea | |
N-bromosuccinimide | completely inhibitory | Phlebiopsis gigantea | |
p-chloromercuribenzoate | completely inhibits at 1 mM | Phlebiopsis gigantea | |
reduced glutathione | 10% inhibition at 1 mM, 95% inhibition at 10 mM | Phlebiopsis gigantea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
oxygen | in the presence of glucose | Phlebiopsis gigantea | |
1.1 | - |
D-glucose | - |
Phlebiopsis gigantea | |
29.4 | - |
D-xylose | - |
Phlebiopsis gigantea | |
50 | - |
L-sorbose | - |
Phlebiopsis gigantea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea | |
Co2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea | |
Mg2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea | |
Zn2+ | slightly stimulates at 1 mM | Phlebiopsis gigantea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
4 * 76000, SDS-PAGE | Phlebiopsis gigantea |
322800 | - |
determined on the basis of its Stokes radius and sedimentation coefficient, dynamic light-scattering experiments, gradient-gel electrophoresis and cross-linking studies | Phlebiopsis gigantea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phlebiopsis gigantea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using fractionated ammonium sulfate precipitation, chromatography on DEAE-Sephacel, Sephacryl S 300, S Sepharose and Q Sepharose | Phlebiopsis gigantea |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.4 | - |
- |
Phlebiopsis gigantea |
Storage Stability | Organism |
---|---|
repeated freezing at -20°C and thawing results in a rapid and irreversible loss of activity | Phlebiopsis gigantea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-melibiose + O2 | 1.9% relative activity to D-glucose | Phlebiopsis gigantea | ? + H2O2 | - |
? | |
D-fucose + O2 | 2.9% relative activity to D-glucose | Phlebiopsis gigantea | ? | - |
? | |
D-galactose + O2 | 7.7% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-gluconolactone + O2 | 1.5% relative activity to D-glucose | Phlebiopsis gigantea | ? + H2O2 | - |
? | |
D-glucose + O2 | activation energy for the conversion of D-glucose is 34.6 kJ/mol | Phlebiopsis gigantea | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | D-glucose is the preferred substrate | Phlebiopsis gigantea | D-glucosone + H2O2 | - |
? | |
D-xylose + O2 | 50% relative activity to D-glucose | Phlebiopsis gigantea | D-xylosone + H2O2 | - |
? | |
L-sorbose + O2 | 109% relative activity to D-glucose | Phlebiopsis gigantea | 5-dehydro-D-fructose + H2O2 | - |
? | |
maltose + O2 | 8.2% relative activity to D-glucose | Phlebiopsis gigantea | ? + H2O2 | - |
? | |
methyl alpha-D-glucoside + O2 | 1.9% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-alpha-D-methylglucoside + H2O2 | - |
? | |
methyl beta-D-glucoside + O2 | 9.6% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-beta-D-methylglucoside + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 76000, SDS-PAGE | Phlebiopsis gigantea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Phlebiopsis gigantea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Phlebiopsis gigantea | |
FAD | 3.6 molecules FAD per molecule enzyme | Phlebiopsis gigantea |