Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Basidiomycota | |
1,10-phenanthroline | 13.3% inhibition | Trametes versicolor | |
8-hydroxyquinoline | - |
Basidiomycota | |
8-hydroxyquinoline | 13.3% inhibition | Trametes versicolor | |
Ag+ | - |
Basidiomycota | |
Ag+ | 53.3% inhibition | Trametes versicolor | |
alpha,alpha'-dipyridyl | 6.1% inhibition | Trametes versicolor | |
Co2+ | 13.3% inhibition | Trametes versicolor | |
Cu2+ | - |
Basidiomycota | |
Cu2+ | 35.7% inhibition | Trametes versicolor | |
H2O2 | 20% inhibition | Trametes versicolor | |
Ni2+ | 28.6% inhibition | Trametes versicolor | |
p-chloromercuribenzoate | 50% inhibition | Trametes versicolor | |
Zn2+ | 13.3% inhibition | Trametes versicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
D-glucose | - |
Trametes versicolor | |
1 | - |
D-glucose | - |
Lenzites styracinus | |
1.7 | - |
D-glucose | - |
Trametes hirsuta | |
1.8 | - |
D-glucose | - |
Gloeophyllum sepiarium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
4 * 68000, SDS-PAGE | Trametes versicolor |
220000 | - |
gel filtration | Trametes versicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Basidiomycota | - |
unidentified | - |
Gloeophyllum sepiarium | - |
- |
- |
Lenzites styracinus | - |
- |
- |
Trametes hirsuta | - |
- |
- |
Trametes versicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography on HPA-75, Sepharose 4B, Sephadex G-100 gel filtration and hydroxylapatite chromatography | Trametes versicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.9 | - |
- |
Trametes versicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | 0.9% relative activity to D-glucose | Trametes versicolor | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + O2 | - |
Trametes hirsuta | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | - |
Lenzites styracinus | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | - |
Gloeophyllum sepiarium | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | other electron acceptors: 2,6-dichlorophenolindophenol, cytochrome c | Trametes versicolor | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | D-glucose is the preferred substrate | Trametes versicolor | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | D-glucose is the preferred substrate | Basidiomycota | D-glucosone + H2O2 | - |
? | |
D-mannose + O2 | 0.9% relative activity to D-glucose | Trametes versicolor | 2-keto-D-mannose + H2O2 | - |
? | |
D-xylose + O2 | - |
Basidiomycota | D-xylosone + H2O2 | - |
? | |
D-xylose + O2 | 2.6% relative activity to D-glucose | Trametes versicolor | D-xylosone + H2O2 | - |
? | |
L-sorbose + O2 | - |
Basidiomycota | 5-dehydro-D-fructose + H2O2 | - |
? | |
L-sorbose + O2 | 3.4% relative activity to D-glucose | Trametes versicolor | 5-dehydro-D-fructose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Basidiomycota |
tetramer | 4 * 68000, SDS-PAGE | Trametes versicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trametes versicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Basidiomycota |
60 | - |
90% of its activity remains after 30 min | Trametes versicolor |
70 | - |
- |
Basidiomycota |
70 | - |
not stable above | Trametes versicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Trametes versicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7.4 | when kept at 50°C for 30 min | Trametes versicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Trametes versicolor | |
FAD | covalently bound | Basidiomycota | |
FAD | covalently bound | Trametes hirsuta | |
FAD | covalently bound | Lenzites styracinus | |
FAD | covalently bound | Gloeophyllum sepiarium |