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Literature summary for 1.1.2.9 extracted from
- An inducible 1-butanol dehydrogenase, a quinohaemoprotein, is involved in the oxidation of butane by Pseudomonas butanovora (2001), Microbiology, 147, 745-756.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
butan-1-ol | at pH 8.0 and 25°C | Thauera butanivorans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Thauera butanivorans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | calcium ions (up to 10 mM) increase the enzyme activity substantially | Thauera butanivorans | |
| NH4+ | activity is increased approximately 3fold in the presence of ammonium ions (4 mM) | Thauera butanivorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
1 * 66000, SDS-PAGE | Thauera butanivorans |
| 69000 | - |
gel filtration | Thauera butanivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butan-1-ol + ferricytochrome c | Thauera butanivorans | - |
butanal + ferrocytochrome c + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thauera butanivorans | - |
- |
- |
| Thauera butanivorans ATCC 43655 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, 4-amino-butanol affinity column chromatography, and Q-Sepharose column chromatography | Thauera butanivorans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:butane-grown cell | - |
Thauera butanivorans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.13 | - |
crude supernatant, at pH 8.0 and 25°C | Thauera butanivorans |
| 4.8 | - |
after 37fold purification, at pH 8.0 and 25°C | Thauera butanivorans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80, 25 mM Tris-HCl pH 8.0, more than 6 months, no loss of activity | Thauera butanivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-butanediol + ferricytochrome c | 30% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 1,2-propanediol + ferricytochrome c | 4% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 1,3-propanediol + ferricytochrome c | 5% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 1,4-butanediol + ferricytochrome c | 24% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 1,4-pentanediol + ferricytochrome c | 1% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 1-heptanol + ferricytochrome c | 31% activity compared to 1-butan-1-ol | Thauera butanivorans | heptanal + ferrocytochrome c + H+ | - |
? | |
| 1-hexanol + ferricytochrome c | 29% activity compared to 1-butan-1-ol | Thauera butanivorans | hexanal + ferrocytochrome c + H+ | - |
? | |
| 1-nonanol + ferricytochrome c | 9% activity compared to 1-butan-1-ol | Thauera butanivorans | nonanal + ferrocytochrome c + H+ | - |
? | |
| 1-octanol + ferricytochrome c | 19% activity compared to 1-butan-1-ol | Thauera butanivorans | octanal + ferrocytochrome c + H+ | - |
? | |
| 1-pentanol + ferricytochrome c | 45% activity compared to 1-butan-1-ol | Thauera butanivorans | pentanal + ferrocytochrome c + H+ | - |
? | |
| 1-propanol + ferricytochrome c | 66% activity compared to 1-butan-1-ol | Thauera butanivorans | propanal + ferrocytochrome c + H+ | - |
? | |
| 2,3-butanediol + ferricytochrome c | 2% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 2,4-pentanediol + ferricytochrome c | 2% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 2-butanol + ferricytochrome c | 9% activity compared to 1-butan-1-ol | Thauera butanivorans | 2-butanone + ferrocytochrome c + H+ | - |
? | |
| 2-heptanol + ferricytochrome c | 49% activity compared to 1-butan-1-ol | Thauera butanivorans | 2-heptanone + ferrocytochrome c + H+ | - |
? | |
| 2-hexanol + ferricytochrome c | 56% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| 2-nonanol + ferricytochrome c | 35% activity compared to 1-butan-1-ol | Thauera butanivorans | 2-nonanone + ferrocytochrome c + H+ | - |
? | |
| 2-octanol + ferricytochrome c | 42% activity compared to 1-butan-1-ol | Thauera butanivorans | 2-octanone + ferrocytochrome c + H+ | - |
? | |
| 2-pentanol + ferricytochrome c | 68% activity compared to 1-butan-1-ol | Thauera butanivorans | 2-pentanone + ferrocytochrome c + H+ | - |
? | |
| 2-propanol + ferricytochrome c | 52% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| acetaldehyde + ferricytochrome c | 54% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| benzyl alcohol + ferricytochrome c | 11% activity compared to 1-butan-1-ol | Thauera butanivorans | benzaldehyde + ferrocytochrome c + H+ | - |
? | |
| butan-1-ol + ferricytochrome c | - |
Thauera butanivorans | butanal + ferrocytochrome c + H+ | - |
? | |
| butyraldehyde + ferricytochrome c | 78% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| ethanol + ferricytochrome c | 34% activity compared to 1-butan-1-ol | Thauera butanivorans | acetaldehyde + ferrocytochrome c + H+ | - |
? | |
| methanol + ferricytochrome c | 2% activity compared to 1-butan-1-ol | Thauera butanivorans | formaldehyde + ferrocytochrome c + H+ | - |
? | |
| additional information | the enzyme also exhibits ferricyanide-dependent alcohol dehydrogenase activity | Thauera butanivorans | ? | - |
? | |
| phenol + ferricytochrome c | 13% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
| propionaldehyde + ferricytochrome c | 61% activity compared to 1-butan-1-ol | Thauera butanivorans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Thauera butanivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-butanol dehydrogenase | - |
Thauera butanivorans |
| BDH | - |
Thauera butanivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thauera butanivorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | the temperature range of maximum stability of the enzyme is 25-32°C, within which the loss of enzyme activity is insignificant after a 30 min incubation. In contrast, enzyme activity is decreased 77% when incubated at 60°C for the same period of time | Thauera butanivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thauera butanivorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme c | - |
Thauera butanivorans | |
| additional information | NAD+-independent | Thauera butanivorans | |
| pyrroloquinoline quinone | - |
Thauera butanivorans | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 720 | - |
butan-1-ol | at pH 8.0 and 25°C | Thauera butanivorans | |
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Release 2023.1 (January 2023)
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