Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Butylamine | - |
Pseudomonas putida | |
Dimethylamine | - |
Pseudomonas putida | |
dipropylamine | - |
Pseudomonas putida | |
ethylamine | inhibitory at high concentrations | Pseudomonas putida | |
heptylamine | - |
Pseudomonas putida | |
Hexylamine | - |
Pseudomonas putida | |
Isobutylamine | slight activation | Pseudomonas putida | |
methylamine | - |
Pseudomonas putida | |
additional information | the enzyme requires ammonia or primary amines as activators in in vitro assays with artificial electron acceptors, the enzyme is activated by various primary amines, dimethylamine, and trimethylamine. tert-Butylamine is a poor activator. The hydrophobic interaction contributes to the binding of amine activators to the binding site in the enzyme | Pseudomonas putida | |
octylamine | - |
Pseudomonas putida | |
pentylamine | the alcohol oxidation activity of PpADH is strongly enhanced by pentylamine and the affinity for substrates is also improved by pentylamine as an activator, inhibitory at high concentrations | Pseudomonas putida | |
Propylamine | - |
Pseudomonas putida | |
triethylamine | - |
Pseudomonas putida | |
trimethylamine | - |
Pseudomonas putida | |
tripropylamine | - |
Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethylamine | activating at low concentrations, inhibitory at high concentrations | Pseudomonas putida | |
pentylamine | the alcohol oxidation activity of PpADH is strongly enhanced by pentylamine and the affinity for substrates is also improved by pentylamine as an activator, inhibitory at high concentrations | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ethanol | pH 9.0, temperature not specified in the publication, in presence of pentylamine | Pseudomonas putida | |
0.21 | - |
ethanol | pH 9.0, temperature not specified in the publication, in presence of ethylamine | Pseudomonas putida | |
0.35 | - |
Propanol | pH 9.0, temperature not specified in the publication, in presence of pentylamine | Pseudomonas putida | |
0.43 | - |
Propanol | pH 9.0, temperature not specified in the publication, in presence of ethylamine | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas putida | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + 2 cytochrome c | - |
Pseudomonas putida | ethanal + 2 reduced cytochrome c | - |
? | |
ethanol + 2 cytochrome c | - |
Pseudomonas putida KT 2240 | ethanal + 2 reduced cytochrome c | - |
? | |
additional information | enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol | Pseudomonas putida | ? | - |
? | |
additional information | enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol | Pseudomonas putida KT 2240 | ? | - |
? | |
propanol + 2 cytochrome c | - |
Pseudomonas putida | propanal + 2 reduced cytochrome c | - |
? | |
propanol + 2 cytochrome c | - |
Pseudomonas putida KT 2240 | propanal + 2 reduced cytochrome c | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PpADH | - |
Pseudomonas putida |
PQQ-ADH | - |
Pseudomonas putida |
pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.8 | - |
ethanol | pH 9.0, temperature not specified in the publication, in presence of ethylamine | Pseudomonas putida | |
32.6 | - |
Propanol | pH 9.0, temperature not specified in the publication, in presence of ethylamine | Pseudomonas putida | |
56.6 | - |
ethanol | pH 9.0, temperature not specified in the publication, in presence of pentylamine | Pseudomonas putida | |
73.1 | - |
Propanol | pH 9.0, temperature not specified in the publication, in presence of pentylamine | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | natural electron acceptor | Pseudomonas putida | |
additional information | enzyme assays are performed with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol | Pseudomonas putida | |
pyrroloquinoline quinone | prosthetic group, dependent on | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
37.6 | - |
pentylamine | pH 9.0, temperature not specified in the publication | Pseudomonas putida | |
112.5 | - |
ethylamine | pH 9.0, temperature not specified in the publication | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
evolution | ADHs are categorized into three groups (type I, II, and III ADHs) according to their domain structure and localization. Type I ADHs have molecular and enzymatic properties that are very similar to those of methanol dehydrogenases, MDHs, but they have a low affinity for methanol. Type I ADHs, on the other hand, generally use ethylamine or methylamine as essential activators instead of ammonia | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73 | - |
Propanol | pH 9.0, temperature not specified in the publication, in presence of ethylamine | Pseudomonas putida | |
115 | - |
ethanol | pH 9.0, temperature not specified in the publication, in presence of ethylamine | Pseudomonas putida | |
209 | - |
Propanol | pH 9.0, temperature not specified in the publication, in presence of pentylamine | Pseudomonas putida | |
395 | - |
ethanol | pH 9.0, temperature not specified in the publication, in presence of pentylamine | Pseudomonas putida |