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Literature summary for 1.1.2.7 extracted from

  • Ghosh, M.; Anthony, C.; Harlos, K.; Goodwin, M.G.; Blake, C.
    The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A (1995), Structure, 3, 177-187.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray diffraction structure determination and analysis at 1.94 A resolution Methylorubrum extorquens

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Methylorubrum extorquens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity, one of the carbonyl oxygens of PQQ is bonded to the Ca2+, probably facilitating attack on the substrate, and the other carbonyl oxygen is out of the plane of the ring, confirming the presence of the predicted free-radical semiquinone form of the prosthetic group Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + ferricytochrome cL Methylorubrum extorquens
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formaldehyde + ferrocytochrome cL
-
?

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens P16027 and P14775 P16027 (large subunit, alpha) and P14775 (small subunit, beta)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + ferricytochrome cL
-
Methylorubrum extorquens formaldehyde + ferrocytochrome cL
-
?
additional information active site structure with important disulfide bridge of Cys103-Cys104, overview Methylorubrum extorquens ?
-
?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, the alpha-subunit has an 8fold radial symmetry, with its eight 3-sheets stabilized by a novel tryptophan docking motif. The PQQ in the active site is held in place by a coplanar tryptophan and by a novel disulfide ring formed between adjacent cysteines which are bonded by an unusual non-planar trans peptide bond, subunit structures and interactions, overview Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
quinoprotein methanol dehydrogenase
-
Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone dependent on, prosthetic group, the PQQ in the active site is held in place by a coplanar tryptophan and by a novel disulfide ring formed between adjacent cysteines which are bonded by an unusual non-planar trans peptide bond. One of the carbonyl oxygens of PQQ is bonded to the Ca2+ , probably facilitating attack on the substrate, and the other carbonyl oxygen is out of the plane of the ring, confirming the presence of the predicted free-radical semiquinone form of the prosthetic group Methylorubrum extorquens