Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4Cl | essential activator | Methyloversatilis universalis | |
NH4Cl | essential activator | Methylibium petroleiphilum |
Cloned (Comment) | Organism |
---|---|
gene mdh2, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Methyloversatilis universalis |
gene mdh2, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Methylibium petroleiphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
ethanol | pH 7.5, 22°C | Methylibium petroleiphilum | |
0.01 | - |
methanol | pH 7.5, 22°C | Methyloversatilis universalis | |
0.01 | - |
n-butanol | pH 7.5, 22°C | Methylibium petroleiphilum | |
0.05 | - |
ethanol | pH 7.5, 22°C | Methyloversatilis universalis | |
0.09 | - |
n-Propanol | pH 7.5, 22°C | Methylibium petroleiphilum | |
0.29 | - |
methanol | pH 7.5, 22°C | Methylibium petroleiphilum | |
0.5 | - |
n-butanol | pH 7.5, 22°C | Methyloversatilis universalis | |
0.5 | - |
n-Propanol | pH 7.5, 22°C | Methyloversatilis universalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Methyloversatilis universalis | - |
- |
periplasm | - |
Methylibium petroleiphilum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methyloversatilis universalis | methanol dehydrogenase is a key enzyme in utilization of methane and methanol by methylotrophic proteobacteria | ? | - |
? | |
additional information | Methylibium petroleiphilum | methanol dehydrogenase is a key enzyme in utilization of methane and methanol by methylotrophic proteobacteria | ? | - |
? | |
additional information | Methyloversatilis universalis FAM5 | methanol dehydrogenase is a key enzyme in utilization of methane and methanol by methylotrophic proteobacteria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylibium petroleiphilum | - |
gene mdh2, the organism lacks gene mxaFI, which encodes the wide-spread dimeric methanol dehydrogenase | - |
Methyloversatilis universalis | - |
gene mdh2, the organism lacks gene mxaFI, which encodes the wide-spread dimeric methanol dehydrogenase | - |
Methyloversatilis universalis FAM5 | - |
gene mdh2, the organism lacks gene mxaFI, which encodes the wide-spread dimeric methanol dehydrogenase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
ethanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methylibium petroleiphilum | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
ethanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis FAM5 | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
methanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis | formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
methanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methylibium petroleiphilum | formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
methanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis FAM5 | formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | methanol dehydrogenase is a key enzyme in utilization of methane and methanol by methylotrophic proteobacteria | Methyloversatilis universalis | ? | - |
? | |
additional information | methanol dehydrogenase is a key enzyme in utilization of methane and methanol by methylotrophic proteobacteria | Methylibium petroleiphilum | ? | - |
? | |
additional information | methanol dehydrogenase is a key enzyme in utilization of methane and methanol by methylotrophic proteobacteria | Methyloversatilis universalis FAM5 | ? | - |
? | |
n-butanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis | butyraldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
n-butanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methylibium petroleiphilum | butyraldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
n-butanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis FAM5 | butyraldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
n-propanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis | propionaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
n-propanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methylibium petroleiphilum | propionaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
n-propanol + 2,6-dichlorophenolindophenol | reduction of the acceptor 2,6-dichlorophenolindophenol is mediated by phenazine methosulfate | Methyloversatilis universalis FAM5 | propionaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | in contrast to other two-subunit pyrroloquinoline quinone-dependent quinoprotein methanol dehydrogenases, wide-spread in Burkholderiales, the enzyme from Methyloversatilis universalis strain FAM5 is a monosubunit protein | Methyloversatilis universalis |
More | in contrast to other two-subunit pyrroloquinoline quinone-dependent quinoprotein methanol dehydrogenases, wide-spread in Burkholderiales, the enzyme from Methylibium petroleiphilum strain PM1 is a monosubunit protein | Methylibium petroleiphilum |
Synonyms | Comment | Organism |
---|---|---|
MDH2 | Mdh2 is a type I alcohol dehydrogenase | Methylibium petroleiphilum |
More | Mdh2 is a type I alcohol dehydrogenase | Methyloversatilis universalis |
pyrroloquinoline quinone-dependent quinoprotein methanol dehydrogenase | - |
Methyloversatilis universalis |
pyrroloquinoline quinone-dependent quinoprotein methanol dehydrogenase | - |
Methylibium petroleiphilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Methyloversatilis universalis |
22 | - |
assay at room temperature | Methylibium petroleiphilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methyloversatilis universalis |
7.5 | - |
assay at | Methylibium petroleiphilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
phenazine methosulfate | mediates reduction of 2,6-dichlorophenol-indophenol | Methyloversatilis universalis | |
phenazine methosulfate | mediates reduction of 2,6-dichlorophenolindophenol | Methylibium petroleiphilum | |
pyrroloquinoline quinone | dependent on | Methyloversatilis universalis | |
pyrroloquinoline quinone | dependent on | Methylibium petroleiphilum |