Protein Variants | Comment | Organism |
---|---|---|
additional information | a Ca2+-free enzyme mxaA mutant is inactive | Methylorubrum extorquens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | one molecule per enzyme alpha-subunit. Neither the quinone form of pyrroloquinoline quinone, nor the disulfide ring or its reduced form are absolutely essential for calcium incorporation into the active site | Methylorubrum extorquens | |
Fe2+ | a cytochrome protein | Methylorubrum extorquens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | P16027 and P14775 | P16027 (large subunit, alpha) and P14775 (small subunit, beta) | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | P16027 and P14775 | P16027 (large subunit, alpha) and P14775 (small subunit, beta) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + ferricytochrome cL | the reaction includes an electron transfer from the quinol PQQH2 to the electron acceptor cytochrome CL mediated by the disulfide structure of the active site, the active site of MDH involves Trp243 that forms the base of the active site chamber, and the Cys103-Cys104 disulfide ring and the pyrroloquinoline quinone prosthetic group, which is in the semiquinone form having the oxygen of the C4 carbonyl displaced out of the plane of the ring, overview | Methylorubrum extorquens | formaldehyde + ferrocytochrome cL | - |
? | |
methanol + ferricytochrome cL | the reaction includes an electron transfer from the quinol PQQH2 to the electron acceptor cytochrome CL mediated by the disulfide structure of the active site, the active site of MDH involves Trp243 that forms the base of the active site chamber, and the Cys103-Cys104 disulfide ring and the pyrroloquinoline quinone prosthetic group, which is in the semiquinone form having the oxygen of the C4 carbonyl displaced out of the plane of the ring, overview | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | formaldehyde + ferrocytochrome cL | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methanol dehydrogenase | - |
Methylorubrum extorquens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Methylorubrum extorquens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome cL | - |
Methylorubrum extorquens | |
additional information | an NAD(P)-independent enzyme | Methylorubrum extorquens | |
pyrroloquinoline quinone | PQQ | Methylorubrum extorquens |