General Stability | Organism |
---|---|
EDTA, DTT, and Ca2+ are slightly stabilizing | Methylorubrum extorquens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | bound to the active site, required for catalytic activity | Methylorubrum extorquens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
7500 | - |
x * 62000, alpha-subunit, + x * 7500, beta-subunit, SDS-PAGE | Methylorubrum extorquens |
62000 | - |
x * 62000, alpha-subunit, + x * 7500, beta-subunit, SDS-PAGE | Methylorubrum extorquens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | P16027 and P14775 | P16027 (large subunit, alpha) and P14775 (small subunit, beta) | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | P16027 and P14775 | P16027 (large subunit, alpha) and P14775 (small subunit, beta) | - |
Purification (Comment) | Organism |
---|---|
native enzyme 22fold from strain AM1 in a single cation exchange chromatographical step, followed by ultrafiltration and buffer exchange, over 97% purity | Methylorubrum extorquens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.4 | - |
purified enzyme | Methylorubrum extorquens |
Storage Stability | Organism |
---|---|
-80°C, purified native enzyme, 20 mM phosphate, pH 7.0, negligible loss of activity in 6 months | Methylorubrum extorquens |
4°C, purified native enzyme, 20 mM phosphate, pH 7.0, loss of 80% activity within 1 week | Methylorubrum extorquens |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, alpha-subunit, + x * 7500, beta-subunit, SDS-PAGE | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Methylorubrum extorquens |
methanol dehydrogenase | - |
Methylorubrum extorquens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | i.e. PQQ or 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylaic acid, enzyme-bound to the active site, 1 molecule per alpha-subunit, required for catalytic activity | Methylorubrum extorquens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methylorubrum extorquens | sequence calculation | - |
8.8 |