Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by fast protein liquid chromatography on hydroxylapatite and on AH-Sepharose with covalently bound 4-hydroxy-a cyanocinnamic acid | Saccharomyces cerevisiae |
hydroxylapatite column chromatography and 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.203 | - |
after purification by 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
0.342 | - |
after purification by hydroxylapatite column chromatography and 4-hydroxy-alpha-cyanocinnamic acid-AH-Sepharose column chromatography, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
3.38 | - |
enzyme purified on H-Sepharose with covalently bound 4-hydroxy-alpha cyanocinnamic acid | Saccharomyces cerevisiae |
5.71 | - |
combination of chromatography on hydroxylapatite and H-Sepharose with covalently bound 4-hydroxy-alpha cyanocinnamic acid | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lactate + 2 ferricytochrome | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome | - |
? | |
D-lactate + ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
? | |
additional information | no cross-reactivity with L-lactic acid | Saccharomyces cerevisiae | ? | - |
? |