Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.4 extracted from

  • Olson, S.T.; Massey, V.
    Purification and properties of the flavoenzyme D-lactate dehydrogenase from Megasphaera elsdenii (1979), Biochemistry, 18, 4714-4723.
    View publication on PubMed

General Stability

General Stability Organism
dithiothreitol and bovine serum albumin required for stability Megasphaera elsdenii

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Megasphaera elsdenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
D-lactate
-
Megasphaera elsdenii

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ rapid inactivation, can be restored by addition of dithiothreitol Megasphaera elsdenii
ferricyanide can substitute for cytochrome c Megasphaera elsdenii
Zn2+ required for activity Megasphaera elsdenii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, sedimentation equilibrium centrifugation and SDS-PAGE Megasphaera elsdenii
105000
-
sedimentation equilibrium centrifugation Megasphaera elsdenii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + 2 ferricytochrome c Megasphaera elsdenii
-
pyruvate + ferrocytochrome c
-
?

Organism

Organism UniProt Comment Textmining
Megasphaera elsdenii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Megasphaera elsdenii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.3
-
purified enzyme Megasphaera elsdenii

Storage Stability

Storage Stability Organism
4°C, in presence of dithiothreitol, stable Megasphaera elsdenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + 2 ferricytochrome c
-
Megasphaera elsdenii pyruvate + ferrocytochrome c
-
?
(R)-lactate + ferricytochrome c
-
Megasphaera elsdenii pyruvate + ferrocytochrome c
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, sedimentation equilibrium centrifugation and SDS-PAGE Megasphaera elsdenii

Cofactor

Cofactor Comment Organism Structure
FAD 1 FAD per subunit Megasphaera elsdenii