General Stability | Organism |
---|---|
dithiothreitol and bovine serum albumin required for stability | Megasphaera elsdenii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Megasphaera elsdenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
D-lactate | - |
Megasphaera elsdenii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | rapid inactivation, can be restored by addition of dithiothreitol | Megasphaera elsdenii | |
ferricyanide | can substitute for cytochrome c | Megasphaera elsdenii | |
Zn2+ | required for activity | Megasphaera elsdenii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, sedimentation equilibrium centrifugation and SDS-PAGE | Megasphaera elsdenii |
105000 | - |
sedimentation equilibrium centrifugation | Megasphaera elsdenii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | Megasphaera elsdenii | - |
pyruvate + ferrocytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megasphaera elsdenii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Megasphaera elsdenii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.3 | - |
purified enzyme | Megasphaera elsdenii |
Storage Stability | Organism |
---|---|
4°C, in presence of dithiothreitol, stable | Megasphaera elsdenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | - |
Megasphaera elsdenii | pyruvate + ferrocytochrome c | - |
? | |
(R)-lactate + ferricytochrome c | - |
Megasphaera elsdenii | pyruvate + ferrocytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, sedimentation equilibrium centrifugation and SDS-PAGE | Megasphaera elsdenii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 FAD per subunit | Megasphaera elsdenii |