Application | Comment | Organism |
---|---|---|
analysis | lactate-selective microbial sensor based on flavocytochrome b2-enriched yeast cells | Ogataea angusta |
Cloned (Comment) | Organism |
---|---|
recombinant overexpression of the enzyme in Hansenula polymorpha yeast cells additionally enriched by recombinant enzyme bound with gold nanoparticles , FC b2-nAu | Ogataea angusta |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a lactate-selective microbial sensor based on flavocytochrome b2-enriched yeast cells. Combination of recombinant technology and nanotechnology by recombinant overexpression of the enzyme in Hansenula polymorpha yeast cells additionally enriched by recombinant enzyme bound with gold nanoparticles, FC b2-nAu. The FC b2-nAu-enriched livingand permeabilized yeast cells are used for construction of a bioselective membrane of microbial L-lactate-selective amperometric biosensor. Phenazine methosulfate serves as a free defusing electron transfer mediator which provides effective electron transfer from the reduced enzyme to the electrode surface. The output to L-lactate of FC b2-nAu-enriched permeabilized yeast cells is 2.5fold higher compared to control cells. For intact cells, the treatment with FC b2-nAu results in 1.56fold increased enzymatic activity. For permeabilized cells, the enrichment efficacy is 2.33fold increased. Method evaluation | Ogataea angusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | W1QKE8 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange chromatography | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
recombinant overexpressed enzyme in crude cell-free extract, pH 7.8, 30°C | Ogataea angusta |
4.45 | - |
recombinant overexpressed enzyme in permeabilized cells, pH 7.8, 30°C | Ogataea angusta |
Subunits | Comment | Organism |
---|---|---|
homotetramer | the enzyme is a tetramer with four identical subunits, each consisting of FMN- and heme-binding domains | Ogataea angusta |
Synonyms | Comment | Organism |
---|---|---|
FC b2 | - |
Ogataea angusta |
flavocytochrome b2 | - |
Ogataea angusta |
L-lactate-cytochrome c-oxidoreductase | - |
Ogataea angusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Ogataea angusta |