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Literature summary for 1.1.2.3 extracted from

  • Mowat, C.G.; Beaudoin, I.; Durley, R.C.; Barton, J.D.; Pike, A.D.; Chen, Z.W.; Reid, G.A.; Chapman, S.K.; Mathews, F.S.; Lederer, F.
    Kinetic and crystallographic studies on the active site Arg289Lys mutant of flavocytochrome b2 (yeast L-lactate dehydrogenase) (2000), Biochemistry, 39, 3266-3275.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli AR120 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of Y143F mutant, vapor diffusion technique in presence of PEG 4000 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A289K mutant Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
D-lactate
-
Saccharomyces cerevisiae
L-Mandelate competitive Saccharomyces cerevisiae
oxalate
-
Saccharomyces cerevisiae
pyruvate competitive at low concentrations, non-competitve at high concentrations Saccharomyces cerevisiae
sulfite competitive Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ferricyanide Y254L mutant Saccharomyces cerevisiae
0.04
-
ferricyanide Y254F mutant Saccharomyces cerevisiae
0.07
-
ferricyanide wild type enzyme Saccharomyces cerevisiae
0.22
-
L-Phenyllactate wild type enzyme Saccharomyces cerevisiae
0.29
-
L-lactate wild type enzyme Saccharomyces cerevisiae
0.34
-
L-lactate Y254F mutant Saccharomyces cerevisiae
0.53
-
L-lactate Y254L mutant Saccharomyces cerevisiae
0.7
-
cytochrome c Y254L mutant Saccharomyces cerevisiae
1.43
-
L-Phenyllactate R289K mutant enzyme Saccharomyces cerevisiae
2.4
-
cytochrome c Y254F mutant Saccharomyces cerevisiae
131
-
cytochrome c wild type enzyme Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial intermembrane space soluble Saccharomyces cerevisiae 5758
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
4 * 57500, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-180°C, long term storage Saccharomyces cerevisiae
-80°C, 0.1 M phosphate buffer, 1 mM EDTA, 1 mM PMSF Saccharomyces cerevisiae
4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + cytochrome c
-
Saccharomyces cerevisiae pyruvate + oxidized cytochrome c
-
?
(S)-mandelate + ferricytochrome c
-
Saccharomyces cerevisiae hydroxy(phenyl)acetate + ferrocytochrome c
-
?
(S)-phenyllactate + ferricytochrome c
-
Saccharomyces cerevisiae phenylpyruvate + ferrocytochrome c
-
?
L-lactate + ferricyanide
-
Saccharomyces cerevisiae pyruvate + ferrocyanide + H+
-
?
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae ?
-
?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 57500, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
L-lactate Y254L mutant Saccharomyces cerevisiae
7.5
-
L-lactate Y254F mutant Saccharomyces cerevisiae
155
-
L-lactate wild type enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Saccharomyces cerevisiae
FMN one FMN per subunit Saccharomyces cerevisiae
heme one heme per subunit Saccharomyces cerevisiae
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0014
-
sulfite
-
Saccharomyces cerevisiae
0.0026
-
L-Mandelate
-
Saccharomyces cerevisiae
0.3
-
oxalate
-
Saccharomyces cerevisiae
1.4
-
D-lactate
-
Saccharomyces cerevisiae
3
-
pyruvate competitive Saccharomyces cerevisiae
30
-
pyruvate non-competitive Saccharomyces cerevisiae