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Literature summary for 1.1.2.3 extracted from

  • Lederer, F.
    On some aspects of the catalysis of lactate dehydrogenation by flavocytochrome c (1991), Flavins and Flavoproteins (Proc. Int. Symp. , 10th, Meeting Date 1990, Curti, B. , Ronchi S. , Zanetti, G. , eds. ) de Gruyter, Berlin, New York, , 773-782.
No PubMed abstract available
Search for more literature for 1.1.2.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
R376K mutant enzyme shows no activity Saccharomyces cerevisiae
Y254F significantly reduced activity of mutant enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.084
-
 Phenyllactate Y254F mutant Saccharomyces cerevisiae
0.35
-
 L-lactate Y254F mutant Saccharomyces cerevisiae
0.4
-
 Phenyllactate wild type enzyme Saccharomyces cerevisiae
0.49
-
 L-lactate wild type enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?
phenyllactate + ferricytochrome c
-
 Saccharomyces cerevisiae phenylpyruvate + ferrocytochrome c
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 Phenyllactate Y254F mutant enzyme Saccharomyces cerevisiae
6.1
-
 L-lactate Y254F mutant enzyme Saccharomyces cerevisiae
16.5
-
 Phenyllactate wild type enzyme Saccharomyces cerevisiae
270
-
 L-lactate wild type enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Saccharomyces cerevisiae
heme
-
 Saccharomyces cerevisiae
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae