Application | Comment | Organism |
---|---|---|
pharmacology | stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals | Microbacterium luteolum |
synthesis | stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals | Microbacterium luteolum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The gene is fused with the His6 tag and thrombin recognition sequence at the N-terminus under the control of the T7 promoter, 3-quinuclidinone reductase BacC | Microbacterium luteolum |
expression in Escherichia coli. The gene is fused with the His6 tag and thrombin recognition sequence at the N-terminus under the control of the T7 promoter, 3-quinuclidinone reductase QNR | Microbacterium luteolum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADH | pH 7.0, 25°C, 3-quinuclidinone reductase QNR | Microbacterium luteolum | |
0.03 | - |
NADH | pH 7.0, 25°C, 3-quinuclidinone reductase BacC | Microbacterium luteolum | |
6.5 | - |
3-quinuclidinone | pH 7.0, 25°C, 3-quinuclidinone reductase QNR | Microbacterium luteolum | |
13.8 | - |
3-quinuclidinone | pH 7.0, 25°C, 3-quinuclidinone reductase BacC | Microbacterium luteolum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30600 | - |
3 * 30600, SDS-PAGE, 3-quinuclidinone reductase QNR | Microbacterium luteolum |
32600 | - |
2 * 32600, SDS-PAGE, 3-quinuclidinone reductase BacC | Microbacterium luteolum |
72000 | - |
gel filtration, 3-quinuclidinone reductase BacC | Microbacterium luteolum |
94000 | - |
gel filtration, 3-quinuclidinone reductase QNR | Microbacterium luteolum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium luteolum | L8B2H6 | - |
- |
Microbacterium luteolum JCM 9174 | L8B2H6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Microbacterium luteolum |
3-quinuclidinone reductase QNR | Microbacterium luteolum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
pH 7.0, 25°C, 3-quinuclidinone reductase BacC | Microbacterium luteolum |
8.4 | - |
pH 7.0, 25°C | Microbacterium luteolum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetylpyridine + NADH + H+ | activity is 16.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC | Microbacterium luteolum | ? | - |
? | |
2-acetylpyridine + NADH + H+ | activity is 16.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC | Microbacterium luteolum JCM 9174 | ? | - |
? | |
3-quinuclidinone + NADH + H+ | 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 100% by 3-quinuclidinone reductase QNR. The optical purity is above 99.9%. No activity with NADPH. The enzyme is strictly specific for 3-quinuclidinone and shows no activity toward several ketones, including tropinone | Microbacterium luteolum | (R)-3-quinuclidinol + NAD+ | - |
? | |
3-quinuclidinone + NADH + H+ | 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 94% bei 3-quinuclidinone reductase BacC. The optical purity is above 99.9%. No activity with NADPH | Microbacterium luteolum | (R)-3-quinuclidinol + NAD+ | - |
? | |
3-quinuclidinone + NADH + H+ | 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 100% by 3-quinuclidinone reductase QNR. The optical purity is above 99.9%. No activity with NADPH. The enzyme is strictly specific for 3-quinuclidinone and shows no activity toward several ketones, including tropinone | Microbacterium luteolum JCM 9174 | (R)-3-quinuclidinol + NAD+ | - |
? | |
3-quinuclidinone + NADH + H+ | 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 94% bei 3-quinuclidinone reductase BacC. The optical purity is above 99.9%. No activity with NADPH | Microbacterium luteolum JCM 9174 | (R)-3-quinuclidinol + NAD+ | - |
? | |
7-oxabicyclo[4.1.0]heptan-2-one + NADH + H+ | activity is 27.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC | Microbacterium luteolum | ? | - |
? | |
7-oxabicyclo[4.1.0]heptan-2-one + NADH + H+ | activity is 27.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC | Microbacterium luteolum JCM 9174 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32600, SDS-PAGE, 3-quinuclidinone reductase BacC | Microbacterium luteolum |
trimer | 3 * 30600, SDS-PAGE, 3-quinuclidinone reductase QNR | Microbacterium luteolum |
Synonyms | Comment | Organism |
---|---|---|
BacC | - |
Microbacterium luteolum |
BacC | bacC isolated from Microbacterium luteolum shows 99.9% identity (100% amino acid identity) with the putative oxidoreductase gene bacC of the bacilysin biosynthetic gene cluster derived from Bacillus subtilis, suggesting that bacC is horizontally transferred from Bacillus species | Microbacterium luteolum |
QNR | - |
Microbacterium luteolum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Microbacterium luteolum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 3-quinuclidinone reductase QNR | Microbacterium luteolum |
7 | - |
3-quinuclidinone reductase BacC | Microbacterium luteolum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | no activity with NADPH, 3-quinuclidinone reductase BacC | Microbacterium luteolum | |
NADH | no activity with NADPH, 3-quinuclidinone reductase QNR | Microbacterium luteolum |