Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rhodotorula mucilaginosa |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour-diffusion method using PEG 8000 as the precipitant. The crystals belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 91.3, c = 265.4 A, and diffracted X-rays to 2.2 A resolution.The asymmetric unit contained four molecules of the protein and the solvent content was 48.4% | Rhodotorula mucilaginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula mucilaginosa | B9ZZZ6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity and ion-exchange column chromatography | Rhodotorula mucilaginosa |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystallographic data | Rhodotorula mucilaginosa |