Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is an important therapeutic target for diabetes in humans | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, 25 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 0.1& Triton, 5% glycerol, and 2 mM EDTA, mixed with equal volume of well solution containing 1.8 M Li2O4, and 0.1 M HEOES, pH 7.5, at room temperature, derivatization with 1 mM ethylmercuric chloride, soaking of crystals with NADH and corticoserone containing solution, X-ray diffraction structure determination and analysis at 3.0 A resolution | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isozymes 11beta-HSD1 and 11beta-HSD2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an 11beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH + H+ | substrate binding site structure, reaction mechanism involving Lys187 and Tyr183, active site structure involving a S-Y-K catalytic triad | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
corticosterone + NAD+ | isozyme 11beta-HSD2 catalyzes the reverse reaction with cofactor NAD+ | Mus musculus | 11-dehydrocorticosterone + NADH + H+ | - |
ir | |
cortisol + NAD+ | isozyme 11beta-HSD2 catalyzes the reverse reaction with cofactor NAD+ | Mus musculus | cortisone + NADH | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD2 | - |
Mus musculus |
11beta-hydroxysteroid dehydrogenase 2 | - |
Mus musculus |