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Literature summary for 1.1.1.B4 extracted from

  • Wang, N.Q.; Sun, J.; Huang, J.; Wang, P.
    Cloning, expression, and directed evolution of carbonyl reductase from Leifsonia xyli HS0904 with enhanced catalytic efficiency (2014), Appl. Microbiol. Biotechnol., 98, 8591-8601.
    View publication on PubMed

Application

Application Comment Organism
synthesis using recombinant Escherichia coli cells expressing Sdr, a yield of 82.5% for (R)-[3,5-bis(trifluoromethyl)phenyl]ethanol can be achieved within 12 h at a substrate concentration of up to 1000 M Leifsonia xyli

Protein Variants

Protein Variants Comment Organism
S154Y mutant exhibits nearly 13fold, 5.4fold, and 2.3fold increase in kcat/Km value, kcat value, and specific activity toward 3,5-bis(trifluoromethyl)acetophenone Leifsonia xyli
S154Y/L194I 180% of wild-type activity Leifsonia xyli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADH wild-type, pH 7.2, 30°C Leifsonia xyli
0.026
-
NADH mutant S154Y/L194I, pH 7.2, 30°C Leifsonia xyli
0.029
-
NADH mutant S154Y, pH 7.2, 30°C Leifsonia xyli
0.2
-
3,5-bis(trifluoromethyl)acetophenone mutant S154Y, pH 7.2, 30°C Leifsonia xyli
0.32
-
3,5-bis(trifluoromethyl)acetophenone mutant S154Y/L194I, pH 7.2, 30°C Leifsonia xyli
0.57
-
3,5-bis(trifluoromethyl)acetophenone wild-type, pH 7.2, 30°C Leifsonia xyli

Organism

Organism UniProt Comment Textmining
Leifsonia xyli T2FLN4
-
-
Leifsonia xyli HS0904 T2FLN4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-bis(trifluoromethyl)acetophenone + NADH + H+
-
Leifsonia xyli (R)-[3,5-bis(trifluoromethyl)phenyl]ethanol + NAD+
-
?
3,5-bis(trifluoromethyl)acetophenone + NADH + H+
-
Leifsonia xyli HS0904 (R)-[3,5-bis(trifluoromethyl)phenyl]ethanol + NAD+
-
?

Synonyms

Synonyms Comment Organism
SDR
-
Leifsonia xyli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.1
-
3,5-bis(trifluoromethyl)acetophenone wild-type, pH 7.2, 30°C Leifsonia xyli
23.6
-
NADH wild-type, pH 7.2, 30°C Leifsonia xyli
26.6
-
NADH mutant S154Y, pH 7.2, 30°C Leifsonia xyli
89.2
-
3,5-bis(trifluoromethyl)acetophenone mutant S154Y/L194I, pH 7.2, 30°C Leifsonia xyli
100
-
NADH mutant S154Y/L194I, pH 7.2, 30°C Leifsonia xyli
114
-
3,5-bis(trifluoromethyl)acetophenone mutant S154Y, pH 7.2, 30°C Leifsonia xyli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
36.8
-
3,5-bis(trifluoromethyl)acetophenone wild-type, pH 7.2, 30°C Leifsonia xyli
277
-
3,5-bis(trifluoromethyl)acetophenone mutant S154Y/L194I, pH 7.2, 30°C Leifsonia xyli
463
-
3,5-bis(trifluoromethyl)acetophenone mutant S154Y, pH 7.2, 30°C Leifsonia xyli
944
-
NADH wild-type, pH 7.2, 30°C Leifsonia xyli
3846
-
NADH mutant S154Y/L194I, pH 7.2, 30°C Leifsonia xyli
4207
-
NADH mutant S154Y, pH 7.2, 30°C Leifsonia xyli