Application | Comment | Organism |
---|---|---|
synthesis | using recombinant Escherichia coli cells expressing Sdr, a yield of 82.5% for (R)-[3,5-bis(trifluoromethyl)phenyl]ethanol can be achieved within 12 h at a substrate concentration of up to 1000 M | Leifsonia xyli |
Protein Variants | Comment | Organism |
---|---|---|
S154Y | mutant exhibits nearly 13fold, 5.4fold, and 2.3fold increase in kcat/Km value, kcat value, and specific activity toward 3,5-bis(trifluoromethyl)acetophenone | Leifsonia xyli |
S154Y/L194I | 180% of wild-type activity | Leifsonia xyli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADH | wild-type, pH 7.2, 30°C | Leifsonia xyli | |
0.026 | - |
NADH | mutant S154Y/L194I, pH 7.2, 30°C | Leifsonia xyli | |
0.029 | - |
NADH | mutant S154Y, pH 7.2, 30°C | Leifsonia xyli | |
0.2 | - |
3,5-bis(trifluoromethyl)acetophenone | mutant S154Y, pH 7.2, 30°C | Leifsonia xyli | |
0.32 | - |
3,5-bis(trifluoromethyl)acetophenone | mutant S154Y/L194I, pH 7.2, 30°C | Leifsonia xyli | |
0.57 | - |
3,5-bis(trifluoromethyl)acetophenone | wild-type, pH 7.2, 30°C | Leifsonia xyli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leifsonia xyli | T2FLN4 | - |
- |
Leifsonia xyli HS0904 | T2FLN4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-bis(trifluoromethyl)acetophenone + NADH + H+ | - |
Leifsonia xyli | (R)-[3,5-bis(trifluoromethyl)phenyl]ethanol + NAD+ | - |
? | |
3,5-bis(trifluoromethyl)acetophenone + NADH + H+ | - |
Leifsonia xyli HS0904 | (R)-[3,5-bis(trifluoromethyl)phenyl]ethanol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SDR | - |
Leifsonia xyli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.1 | - |
3,5-bis(trifluoromethyl)acetophenone | wild-type, pH 7.2, 30°C | Leifsonia xyli | |
23.6 | - |
NADH | wild-type, pH 7.2, 30°C | Leifsonia xyli | |
26.6 | - |
NADH | mutant S154Y, pH 7.2, 30°C | Leifsonia xyli | |
89.2 | - |
3,5-bis(trifluoromethyl)acetophenone | mutant S154Y/L194I, pH 7.2, 30°C | Leifsonia xyli | |
100 | - |
NADH | mutant S154Y/L194I, pH 7.2, 30°C | Leifsonia xyli | |
114 | - |
3,5-bis(trifluoromethyl)acetophenone | mutant S154Y, pH 7.2, 30°C | Leifsonia xyli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.8 | - |
3,5-bis(trifluoromethyl)acetophenone | wild-type, pH 7.2, 30°C | Leifsonia xyli | |
277 | - |
3,5-bis(trifluoromethyl)acetophenone | mutant S154Y/L194I, pH 7.2, 30°C | Leifsonia xyli | |
463 | - |
3,5-bis(trifluoromethyl)acetophenone | mutant S154Y, pH 7.2, 30°C | Leifsonia xyli | |
944 | - |
NADH | wild-type, pH 7.2, 30°C | Leifsonia xyli | |
3846 | - |
NADH | mutant S154Y/L194I, pH 7.2, 30°C | Leifsonia xyli | |
4207 | - |
NADH | mutant S154Y, pH 7.2, 30°C | Leifsonia xyli |