Application | Comment | Organism |
---|---|---|
synthesis | ethyl benzoylformate is asymmetrically reduced by the purified enzyme, using an additional coupled NADH regeneration system, with 95% conversion and in an enantiomeric excess of 99.9% | Carboxydothermus hydrogenoformans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Carboxydothermus hydrogenoformans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.3 | - |
ethyl 2-oxopropanoate | pH 6.5, 50°C | Carboxydothermus hydrogenoformans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE and calculated | Carboxydothermus hydrogenoformans |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 10% v/v, 30% residual activity | Carboxydothermus hydrogenoformans |
DMSO | 10% v/v, 50% residual activity | Carboxydothermus hydrogenoformans |
Ethanol | 10% v/v, 50% residual activity | Carboxydothermus hydrogenoformans |
tetrahydrofuran | 10% v/v, 25% residual activity | Carboxydothermus hydrogenoformans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carboxydothermus hydrogenoformans | Q3ACV3 | - |
- |
Carboxydothermus hydrogenoformans DSM 6008 | Q3ACV3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylpentan-2-one + NADH + H+ | 46% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | 4-methylpentan-2-ol + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
4-methylpentan-2-one + NADH + H+ | 46% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans DSM 6008 | 4-methylpentan-2-ol + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
cycloheptanone + NADH + H+ | 69% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | cycloheptanol + NAD+ | - |
? | |
cycloheptanone + NADH + H+ | 69% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans DSM 6008 | cycloheptanol + NAD+ | - |
? | |
ethyl 2-oxo-3-phenylpropanoate + NADH + H+ | 48% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | ethyl 2-hydroxy-3-phenylpropanoate + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
ethyl 2-oxo-4-phenylbutanoate + NADH + H+ | 135% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | ethyl 2-hydroxy-4-phenylbutanoate + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
ethyl 2-oxopropanoate + NADH + H+ | - |
Carboxydothermus hydrogenoformans | ethyl 2-hydroxypropanoate + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
ethyl 3-oxohexanoate + NADH + H+ | 105% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | ethyl 3-hydroxyhexanoate + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
ethyl 4-chloro-3-oxobutanoate + NADH + H+ | 129% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | ethyl 4-chloro-3-hydroxybutanoate + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
ethyl 4-chloro-3-oxobutanoate + NADH + H+ | 129% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans DSM 6008 | ethyl 4-chloro-3-hydroxybutanoate + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
ethyl benzoylformate + NADH + H+ | 270% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | ethyl (R)-mandelate + NAD+ | 95% conversion with 99.9% enantiomeric excess | ? | |
ethyl benzoylformate + NADH + H+ | 270% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans DSM 6008 | ethyl (R)-mandelate + NAD+ | 95% conversion with 99.9% enantiomeric excess | ? | |
ethyl oxo(phenyl)acetate + NADH + H+ | 270% of the activity with ethyl 2-oxopropanoate | Carboxydothermus hydrogenoformans | ethyl hydroxy(phenyl)acetate + NAD+ | chirality of the product not determined. The enzyme prouces ethyl (R)-mandelate from ethal benzoylformate | ? | |
additional information | enzyme exhibits reductase activity towards a broad spectrum of substrates including aliphatic linear, branched, and cyclic ketones, aromatic ketones, alpha-ketoesters, and beta-ketoesters. Both alpha- and beta-ketoesters serve as better substrates than ketones, but the enzymes shows a preference for alpha-ketoesters | Carboxydothermus hydrogenoformans | ? | - |
? | |
additional information | enzyme exhibits reductase activity towards a broad spectrum of substrates including aliphatic linear, branched, and cyclic ketones, aromatic ketones, alpha-ketoesters, and beta-ketoesters. Both alpha- and beta-ketoesters serve as better substrates than ketones, but the enzymes shows a preference for alpha-ketoesters | Carboxydothermus hydrogenoformans DSM 6008 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE and calculated | Carboxydothermus hydrogenoformans |
Synonyms | Comment | Organism |
---|---|---|
CHY1186 | - |
Carboxydothermus hydrogenoformans |
SDR | - |
Carboxydothermus hydrogenoformans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Carboxydothermus hydrogenoformans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.97 | - |
ethyl 2-oxopropanoate | pH 6.5, 50°C | Carboxydothermus hydrogenoformans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Carboxydothermus hydrogenoformans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | preferred over NADPH | Carboxydothermus hydrogenoformans | |
NADPH | about 10% of the activity with NADH | Carboxydothermus hydrogenoformans |