Protein Variants | Comment | Organism |
---|---|---|
I86A | site-directed mutagensis, the secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols, in contrast to the wild-type enzyme, the mutation I86A allows large substituents to fit into the large pocket of I86ATeSADH, which corresponds to the small pocket in wild-type TeSADH, modeling of the stereopreference of TeSADH I86A | Thermoanaerobacter ethanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | Thermoanaerobacter ethanolicus | activity of the wild-type enzyme, the mutant I886A is (R)-specific forming (R)-1-phenylethanol | acetophenone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | P77990 | gene adhB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | activity of the wild-type enzyme, the mutant I886A is (R)-specific forming (R)-1-phenylethanol | Thermoanaerobacter ethanolicus | acetophenone + NADH + H+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoanaerobacter ethanolicus | |
NADH | - |
Thermoanaerobacter ethanolicus |