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Literature summary for 1.1.1.B3 extracted from

  • Yamamoto, H.; Matsuyama, A.; Kobayashi, Y.
    Synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate with recombinant Escherichia coli cells expressing (S)-specific secondary alcohol dehydrogenase (2002), Biosci. Biotechnol. Biochem., 66, 481-483.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of ethyl (R)-4-chloro-3-hydroxybutanoate using whole recombinant cells of Escherichia coli and 2-propanol as an energy source to regenerate NADH. Yield reaches 36.6 g/l with purity of more than 99% enantiomeric excess and 95.2% conversion Candida parapsilosis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl 4-chloro-3-oxobutanoate + NADH + H+
-
Candida parapsilosis ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
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r