Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.B20 extracted from

  • Rados, D.; Turner, D.L.; Catarino, T.; Hoffart, E.; Neves, A.R.; Eikmanns, B.J.; Blombach, B.; Santos, H.
    Stereospecificity of Corynebacterium glutamicum 2,3-butanediol dehydrogenase and implications for the stereochemical purity of bioproduced 2,3-butanediol (2016), Appl. Microbiol. Biotechnol., 100, 10573-10583 .
    View publication on PubMed
Search for more literature for 1.1.1.B20

Cloned(Commentary)

Cloned (Comment) Organism
gene butACg, subcloning in Escherichia coli strain DH5alpha, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with the genes encoding ALS and ALDC of Lactobacillus lactis strain MG1363 Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
moe construction and engineering of Corynebacterium glutamicum strain DELTAaceEDELTApqoDELTAldhA(pEKEx2-als,aldB,butACg). Chromosomal inactivation of the putative BDH gene butACg (cg2958) in strain DELTAaceEDELTApqoDELTAldhA. BDH activity is nearly abolished upon inactivation of butACg indicating that Corynebacterium glutamicum expresses a single BDH under the experimental conditions examined. BDH activity increases 3fold in strain DELTAaceEDELTApqoDELTAldhA(pEKEx2-als,aldB,butACg) compared to the respective control. The inactivation of butACg gene decreases the BDH activity 75fold for the DELTAaceEDELTApqoDELTAldhADELTAbutACg(pEKEx2) strain compared to strain DELTAaceEDELTApqoDELTAldhA(pEKEx2). The major form of 2,3-butanediol is meso-2,3-butandediol, and the ratio meso-2,3-BD/optically active 2,3-BD is 95:5, the main side products are glycerol, ethanol, and acetoin Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
(2S,3S)-2,3-butanediol product inhibition Corynebacterium glutamicum
additional information no inhibition by NAD+ at 2 mM and 5 mM Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 (2S,3S)-2,3-butanediol pH 10.5, 30°C, recombinant enzyme Corynebacterium glutamicum
0.23
-
 acetoin racemic (R/S)-acetoin, pH 7.0, 30°C, recombinant enzyme Corynebacterium glutamicum
1.49
-
 (R)-acetoin pH 7.0, 30°C, recombinant enzyme Corynebacterium glutamicum
2.01
-
 meso-2,3-butanediol pH 10.5, 30°C, recombinant enzyme Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 recombinant Escherichia coli strain BL21(DE3) expressing wild-type BDH, pH 7.0, 30°C Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-acetoin + NADH + H+
-
 Corynebacterium glutamicum meso-2,3-butanediol + NAD+
-
 r
(S)-acetoin + NADH + H+
-
 Corynebacterium glutamicum meso-2,3-butanediol + NAD+
-
 r
2 acetoin + 2 NADH + 2 H+ racemic mixture of (3S/3R)-acetoin Corynebacterium glutamicum (2S,3S)-2,3-butanediol + meso-2,3-butanediol + 2 NAD+
-
 r
additional information NMR identification and quantification of reaction products. Meso-2,3-butanediol is the major form (over 95% of the 2,3-butanediol pool, depending on oxygen availability) produced in fermentations using a strain that overexpresses ALS/ALDC of Lactobacillus lactis and BDH of Corynebacterium glutamicum, i.e., strain DELTAaceEDELTApqoDELTAldhA(pEKEx2als,aldB,butACg) Corynebacterium glutamicum ?
-
 ?

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenase
-
 Corynebacterium glutamicum
BDH
-
 Corynebacterium glutamicum
butACg
-
 Corynebacterium glutamicum
meso-2,3-BDH
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 acetoin reduction assay at Corynebacterium glutamicum
10.5
-
 2,3-butanediol oxidation assay at Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Corynebacterium glutamicum
NADH
-
 Corynebacterium glutamicum

General Information

General Information Comment Organism
evolution enzyme BDH belongs to the SDR family, of enzymes Corynebacterium glutamicum
metabolism pathways for the synthesis of 2,3-butanediol in bacteria, overview Corynebacterium glutamicum