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Literature summary for 1.1.1.B18 extracted from

  • Kataoka, M.; Nakamura, Y.; Urano, N.; Ishige, T.; Shi, G.; Kita, S.; Sakamoto, K.; Shimizu, S.
    A novel NADP+-dependent L-1-amino-2-propanol dehydrogenase from Rhodococcus erythropolis MAK154: a promising enzyme for the production of double chiral aminoalcohols (2006), Lett. Appl. Microbiol., 43, 430-455.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
4 * 28500, SDS-PAGE Rhodococcus erythropolis
105000
-
gel filtration Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis A1IG83
-
-
Rhodococcus erythropolis MAK154 A1IG83
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.43
-
pH 7.5, 43°C Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
Rhodococcus erythropolis (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
-
r
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
Rhodococcus erythropolis MAK154 (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
-
r
DL-1-amino-2-butanol + NADPH + H+ 95% of the activity with L-1-amino-2-propanol Rhodococcus erythropolis ? + NADP+
-
?
DL-1-amino-2-butanol + NADPH + H+ 95% of the activity with L-1-amino-2-propanol Rhodococcus erythropolis MAK154 ? + NADP+
-
?
DL-2-aminocyclohexanol + NADPH + H+ 50% of the activity with L-1-amino-2-propanol Rhodococcus erythropolis ? + NADP+
-
?
DL-2-aminocyclohexanol + NADPH + H+ 50% of the activity with L-1-amino-2-propanol Rhodococcus erythropolis MAK154 ? + NADP+
-
?
L-1-amino-2-propanol + NADPH + H+ best substrate Rhodococcus erythropolis ? + NADP+
-
?
L-1-amino-2-propanol + NADPH + H+ best substrate Rhodococcus erythropolis MAK154 ? + NADP+
-
?
additional information no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol Rhodococcus erythropolis ?
-
?
additional information no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol Rhodococcus erythropolis MAK154 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 28500, SDS-PAGE Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
assay at Rhodococcus erythropolis
55
-
-
Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Rhodococcus erythropolis

Expression

Organism Comment Expression
Rhodococcus erythropolis expression is induced on cultivation with aminoalcohols such as 1-amino-2-propanol, 1-amino-2-butanol and 2-aminocyclohexanol up