Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28500 | - |
4 * 28500, SDS-PAGE | Rhodococcus erythropolis |
105000 | - |
gel filtration | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | A1IG83 | - |
- |
Rhodococcus erythropolis MAK154 | A1IG83 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.43 | - |
pH 7.5, 43°C | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+ | - |
Rhodococcus erythropolis | (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ | - |
r | |
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+ | - |
Rhodococcus erythropolis MAK154 | (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ | - |
r | |
DL-1-amino-2-butanol + NADPH + H+ | 95% of the activity with L-1-amino-2-propanol | Rhodococcus erythropolis | ? + NADP+ | - |
? | |
DL-1-amino-2-butanol + NADPH + H+ | 95% of the activity with L-1-amino-2-propanol | Rhodococcus erythropolis MAK154 | ? + NADP+ | - |
? | |
DL-2-aminocyclohexanol + NADPH + H+ | 50% of the activity with L-1-amino-2-propanol | Rhodococcus erythropolis | ? + NADP+ | - |
? | |
DL-2-aminocyclohexanol + NADPH + H+ | 50% of the activity with L-1-amino-2-propanol | Rhodococcus erythropolis MAK154 | ? + NADP+ | - |
? | |
L-1-amino-2-propanol + NADPH + H+ | best substrate | Rhodococcus erythropolis | ? + NADP+ | - |
? | |
L-1-amino-2-propanol + NADPH + H+ | best substrate | Rhodococcus erythropolis MAK154 | ? + NADP+ | - |
? | |
additional information | no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol | Rhodococcus erythropolis | ? | - |
? | |
additional information | no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol | Rhodococcus erythropolis MAK154 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 28500, SDS-PAGE | Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
assay at | Rhodococcus erythropolis |
55 | - |
- |
Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Rhodococcus erythropolis |
Organism | Comment | Expression |
---|---|---|
Rhodococcus erythropolis | expression is induced on cultivation with aminoalcohols such as 1-amino-2-propanol, 1-amino-2-butanol and 2-aminocyclohexanol | up |