Cloned (Comment) | Organism |
---|---|
gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (2S)-2-(methylamino)-1-phenylpropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol | product inhibition above 20 mg/ml | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26580 | - |
x * 26580, calculated | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | A1IG83 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+ | diastereoselective reduction | Rhodococcus erythropolis | (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ | i.e. d-psi-pseudoephedrine, molar reaction yields of 89.1% and more than 99% enantiomeric and diastereomeric excess | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26580, calculated | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
AADH | - |
Rhodococcus erythropolis |
L-1-amino-2-propanol dehydrogenase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodococcus erythropolis |