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Literature summary for 1.1.1.95 extracted from
- Crystal structures and kinetics of Type III 3-phosphoglycerate dehydrogenase reveal catalysis by lysine (2014), FEBS J., 281, 5498-5512.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Entamoeba histolytica |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complexes with 3-phospho-D-glycerate and NAD+, hanging drop vapor diffusion method, using 20% (w/v) poly(ethylene glycol) 3350 in 100 mM Tris pH 7.0-8.5, 350 mM sodium formate and 5% (v/v) glycerol | Entamoeba histolytica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K263A | the mutant shows strongly reduced activity compared to the wild type enzyme | Entamoeba histolytica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.396 | - |
3-phospho-D-glycerate | wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica |  |
| 0.6 | - |
3-phospho-D-glycerate | mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NAD+ | Entamoeba histolytica | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | Q76KF5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-Sepharose column chromatography and Superdex 200 gel filtration | Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.004 | - |
mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica |
| 0.495 | - |
wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NAD+ | - |
Entamoeba histolytica | 3-phosphonooxypyruvate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x-ray crystallography | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGDH | - |
Entamoeba histolytica |
| type III 3-phosphoglycerate dehydrogenase | - |
Entamoeba histolytica |
| type IIIK PGDH | - |
Entamoeba histolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
3-phospho-D-glycerate | mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica | |
| 16.28 | - |
3-phospho-D-glycerate | wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Entamoeba histolytica | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.216 | - |
3-phospho-D-glycerate | mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica | |
| 4.2 | - |
3-phospho-D-glycerate | wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica | |
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