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Literature summary for 1.1.1.95 extracted from

  • Xu, X.L.; Chen, S.; Salinas, N.D.; Tolia, N.H.; Grant, G.A.
    Comparison of type 1 D-3-phosphoglycerate dehydrogenases reveals unique regulation in pathogenic Mycobacteria (2015), Arch. Biochem. Biophys., 570, 32-39.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
expressed in Escherichia coli BL21(DE3) cells Homo sapiens
expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
expressed in Escherichia coli BL21(DE3) cells Mycobacterium marinum
expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
L-serine
-
Bacillus subtilis
L-serine
-
Corynebacterium glutamicum
L-serine
-
Homo sapiens
L-serine
-
Mycobacterium marinum
L-serine
-
Mycobacterium tuberculosis
L-serine
-
Mycolicibacterium smegmatis
L-serine
-
Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
0.004
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
0.008
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
0.014
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
0.015
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
0.025
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.031
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
0.041
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
0.049
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
0.065
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
0.127
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
0.153
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.162
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
0.292
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
-
Streptomyces coelicolor
57200
-
-
Bacillus subtilis
57200
-
-
Homo sapiens
207300
-
analytical ultracentrifugation Bacillus subtilis
207300
-
analytical ultracentrifugation Homo sapiens
207300
-
analytical ultracentrifugation Mycolicibacterium smegmatis
207300
-
analytical ultracentrifugation Corynebacterium glutamicum
207300
-
analytical ultracentrifugation Mycobacterium tuberculosis
207300
-
analytical ultracentrifugation Mycobacterium marinum
207300
-
analytical ultracentrifugation Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glycerate + NAD+ Bacillus subtilis
-
3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+ Homo sapiens
-
3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+ Mycolicibacterium smegmatis
-
3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+ Corynebacterium glutamicum
-
3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+ Mycobacterium tuberculosis
-
3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+ Mycobacterium marinum
-
3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+ Streptomyces coelicolor
-
3-phosphonooxypyruvate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Corynebacterium glutamicum
-
-
-
Homo sapiens
-
-
-
Mycobacterium marinum
-
-
-
Mycobacterium tuberculosis
-
-
-
Mycolicibacterium smegmatis
-
-
-
Streptomyces coelicolor Q9Z564
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon cobalt based immobilized metal affinity column chromatography Bacillus subtilis
Talon cobalt based immobilized metal affinity column chromatography Homo sapiens
Talon cobalt based immobilized metal affinity column chromatography Mycolicibacterium smegmatis
Talon cobalt based immobilized metal affinity column chromatography Corynebacterium glutamicum
Talon cobalt based immobilized metal affinity column chromatography Mycobacterium tuberculosis
Talon cobalt based immobilized metal affinity column chromatography Mycobacterium marinum
Talon cobalt based immobilized metal affinity column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + NAD+
-
Bacillus subtilis 3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+
-
Homo sapiens 3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+
-
Mycolicibacterium smegmatis 3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+
-
Corynebacterium glutamicum 3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+
-
Mycobacterium tuberculosis 3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+
-
Mycobacterium marinum 3-phosphonooxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+
-
Streptomyces coelicolor 3-phosphonooxypyruvate + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 55000, calculated from amino acid sequence Streptomyces coelicolor
homotetramer 4 * 57200, calculated from amino acid sequence Bacillus subtilis
homotetramer 4 * 57200, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
D-3-phosphoglycerate dehydrogenase
-
Bacillus subtilis
D-3-phosphoglycerate dehydrogenase
-
Homo sapiens
D-3-phosphoglycerate dehydrogenase
-
Mycolicibacterium smegmatis
D-3-phosphoglycerate dehydrogenase
-
Corynebacterium glutamicum
D-3-phosphoglycerate dehydrogenase
-
Mycobacterium tuberculosis
D-3-phosphoglycerate dehydrogenase
-
Mycobacterium marinum
D-3-phosphoglycerate dehydrogenase
-
Streptomyces coelicolor
PGDH
-
Bacillus subtilis
PGDH
-
Homo sapiens
PGDH
-
Mycolicibacterium smegmatis
PGDH
-
Corynebacterium glutamicum
PGDH
-
Mycobacterium tuberculosis
PGDH
-
Mycobacterium marinum
PGDH
-
Streptomyces coelicolor
type 1 D-3-phosphoglycerate dehydrogenase
-
Bacillus subtilis
type 1 D-3-phosphoglycerate dehydrogenase
-
Homo sapiens
type 1 D-3-phosphoglycerate dehydrogenase
-
Mycolicibacterium smegmatis
type 1 D-3-phosphoglycerate dehydrogenase
-
Corynebacterium glutamicum
type 1 D-3-phosphoglycerate dehydrogenase
-
Mycobacterium tuberculosis
type 1 D-3-phosphoglycerate dehydrogenase
-
Mycobacterium marinum
type 1 D-3-phosphoglycerate dehydrogenase
-
Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
194
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
198
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
311
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
341
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
350
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
464
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
567
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
576
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
608
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
1515
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1575
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
2425
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
3850
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NAD+
-
Homo sapiens
NAD+
-
Mycolicibacterium smegmatis
NAD+
-
Corynebacterium glutamicum
NAD+
-
Mycobacterium tuberculosis
NAD+
-
Mycobacterium marinum
NAD+
-
Streptomyces coelicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.016
-
L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
0.038
-
L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
0.057
-
L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
0.063
-
L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
0.066
-
L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
0.082
-
L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
0.115
-
L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
0.138
-
L-serine in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
0.255
-
L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
0.581
-
L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
0.628
-
L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
1.065
-
L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
4.539
-
L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
5.898
-
L-serine in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1400
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
2400
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Homo sapiens
3200
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
3600
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Streptomyces coelicolor
5000
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
5300
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Corynebacterium glutamicum
6100
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
7600
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
8300
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium marinum
13200
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycolicibacterium smegmatis
14000
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Homo sapiens
15800
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Mycobacterium tuberculosis
18000
-
3-phospho-D-glycerate in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis
31000
-
3-phospho-D-glycerate in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication Bacillus subtilis