Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
expressed in Escherichia coli BL21(DE3) cells | Mycolicibacterium smegmatis |
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium marinum |
expressed in Escherichia coli BL21(DE3) cells | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-serine | - |
Bacillus subtilis | |
L-serine | - |
Corynebacterium glutamicum | |
L-serine | - |
Homo sapiens | |
L-serine | - |
Mycobacterium marinum | |
L-serine | - |
Mycobacterium tuberculosis | |
L-serine | - |
Mycolicibacterium smegmatis | |
L-serine | - |
Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.004 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
0.008 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
0.014 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
0.015 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis | |
0.025 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.031 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis | |
0.041 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
0.049 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis | |
0.065 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
0.127 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.153 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.162 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
0.292 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
- |
Streptomyces coelicolor |
57200 | - |
- |
Bacillus subtilis |
57200 | - |
- |
Homo sapiens |
207300 | - |
analytical ultracentrifugation | Bacillus subtilis |
207300 | - |
analytical ultracentrifugation | Homo sapiens |
207300 | - |
analytical ultracentrifugation | Mycolicibacterium smegmatis |
207300 | - |
analytical ultracentrifugation | Corynebacterium glutamicum |
207300 | - |
analytical ultracentrifugation | Mycobacterium tuberculosis |
207300 | - |
analytical ultracentrifugation | Mycobacterium marinum |
207300 | - |
analytical ultracentrifugation | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | Bacillus subtilis | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | Homo sapiens | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | Mycolicibacterium smegmatis | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | Corynebacterium glutamicum | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | Mycobacterium tuberculosis | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | Mycobacterium marinum | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | Streptomyces coelicolor | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Corynebacterium glutamicum | - |
- |
- |
Homo sapiens | - |
- |
- |
Mycobacterium marinum | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Streptomyces coelicolor | Q9Z564 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon cobalt based immobilized metal affinity column chromatography | Bacillus subtilis |
Talon cobalt based immobilized metal affinity column chromatography | Homo sapiens |
Talon cobalt based immobilized metal affinity column chromatography | Mycolicibacterium smegmatis |
Talon cobalt based immobilized metal affinity column chromatography | Corynebacterium glutamicum |
Talon cobalt based immobilized metal affinity column chromatography | Mycobacterium tuberculosis |
Talon cobalt based immobilized metal affinity column chromatography | Mycobacterium marinum |
Talon cobalt based immobilized metal affinity column chromatography | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | - |
Bacillus subtilis | 3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Homo sapiens | 3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Mycolicibacterium smegmatis | 3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Corynebacterium glutamicum | 3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Mycobacterium tuberculosis | 3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Mycobacterium marinum | 3-phosphonooxypyruvate + NADH + H+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Streptomyces coelicolor | 3-phosphonooxypyruvate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 55000, calculated from amino acid sequence | Streptomyces coelicolor |
homotetramer | 4 * 57200, calculated from amino acid sequence | Bacillus subtilis |
homotetramer | 4 * 57200, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
D-3-phosphoglycerate dehydrogenase | - |
Bacillus subtilis |
D-3-phosphoglycerate dehydrogenase | - |
Homo sapiens |
D-3-phosphoglycerate dehydrogenase | - |
Mycolicibacterium smegmatis |
D-3-phosphoglycerate dehydrogenase | - |
Corynebacterium glutamicum |
D-3-phosphoglycerate dehydrogenase | - |
Mycobacterium tuberculosis |
D-3-phosphoglycerate dehydrogenase | - |
Mycobacterium marinum |
D-3-phosphoglycerate dehydrogenase | - |
Streptomyces coelicolor |
PGDH | - |
Bacillus subtilis |
PGDH | - |
Homo sapiens |
PGDH | - |
Mycolicibacterium smegmatis |
PGDH | - |
Corynebacterium glutamicum |
PGDH | - |
Mycobacterium tuberculosis |
PGDH | - |
Mycobacterium marinum |
PGDH | - |
Streptomyces coelicolor |
type 1 D-3-phosphoglycerate dehydrogenase | - |
Bacillus subtilis |
type 1 D-3-phosphoglycerate dehydrogenase | - |
Homo sapiens |
type 1 D-3-phosphoglycerate dehydrogenase | - |
Mycolicibacterium smegmatis |
type 1 D-3-phosphoglycerate dehydrogenase | - |
Corynebacterium glutamicum |
type 1 D-3-phosphoglycerate dehydrogenase | - |
Mycobacterium tuberculosis |
type 1 D-3-phosphoglycerate dehydrogenase | - |
Mycobacterium marinum |
type 1 D-3-phosphoglycerate dehydrogenase | - |
Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
194 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
198 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
311 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
341 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
350 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
464 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis | |
567 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis | |
576 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
608 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
1515 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
1575 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis | |
2425 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
3850 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Mycolicibacterium smegmatis | |
NAD+ | - |
Corynebacterium glutamicum | |
NAD+ | - |
Mycobacterium tuberculosis | |
NAD+ | - |
Mycobacterium marinum | |
NAD+ | - |
Streptomyces coelicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
L-serine | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis | |
0.038 | - |
L-serine | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
0.057 | - |
L-serine | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.063 | - |
L-serine | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
0.066 | - |
L-serine | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
0.082 | - |
L-serine | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.115 | - |
L-serine | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
0.138 | - |
L-serine | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis | |
0.255 | - |
L-serine | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis | |
0.581 | - |
L-serine | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
0.628 | - |
L-serine | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
1.065 | - |
L-serine | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
4.539 | - |
L-serine | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis | |
5.898 | - |
L-serine | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1400 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
2400 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
3200 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis | |
3600 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Streptomyces coelicolor | |
5000 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
5300 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Corynebacterium glutamicum | |
6100 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
7600 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
8300 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium marinum | |
13200 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycolicibacterium smegmatis | |
14000 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Homo sapiens | |
15800 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
18000 | - |
3-phospho-D-glycerate | in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis | |
31000 | - |
3-phospho-D-glycerate | in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication | Bacillus subtilis |