Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.95 extracted from

  • Jun, d.o..Y.; Park, H.S.; Lee, J.Y.; Baek, J.Y.; Park, H.K.; Fukui, K.; Kim, Y.H.
    Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y (2008), Gene, 414, 106-114.
    View publication on PubMed

Application

Application Comment Organism
molecular biology results demonstrate that the promoter activity of the human PHGDH gene is positively regulated by the action of transcription factors Sp1 and NF-Y Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information sequence analysis of the PHGDH promoter region exhibits several putative transcription factor binding sites for NF-Y, Sp1, GATA-1, p53, AP2, and AP1, with no TATA-box motif at an appropriate position. Transfection of a series of deletion constructs of the promoter region into HeLa cells reveals that the core positive promoter activity resides in the -276 to +1, which contains two GC-motifs for binding Sp1 and one CCAAT-motif for NF-Y. Mutational analysis and electrophoretic mobility shift assay indicates that both the proximal GC-motif and CCAAT-motif are crucial for full induction of the promoter activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Synonyms

Synonyms Comment Organism
3-phosphoglycerate dehydrogenase
-
Homo sapiens
PGDH
-
Homo sapiens