Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H344A | 50% inhibition at 0.8 mM L-serine | Escherichia coli |
H344A/N364A | no inhibition by L-serine | Escherichia coli |
N346A | 50% inhibition at 6 mM L-serine | Escherichia coli |
N346A/H344A | no inhibition by L-serine | Escherichia coli |
N346A/N364A | no inhibition by L-serine | Escherichia coli |
N364A | 50% inhibition at 48 mM L-serine | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-2-amino-1-propanol | slightly | Escherichia coli | |
amino acids | - |
Escherichia coli | |
beta-Alanine | slightly | Escherichia coli | |
glycine | mutants show less to no inhibition | Escherichia coli | |
L-alanine | native enzyme and mutant H344A | Escherichia coli | |
L-serine | 50% inhibition at 0.008 mM L-serine; allosteric inhibition, regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
alpha-ketoglutarate | apparent, double mutant H344A/N364A | Escherichia coli | |
0.042 | - |
alpha-ketoglutarate | apparent | Escherichia coli | |
0.044 | - |
alpha-ketoglutarate | apparent, mutant N346A | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphoglycerate + NAD+ | Escherichia coli | allosteric inhibition by L-serine, L-serine regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364, 1 serine binds per subunit | 3-phosphohydroxypyruvate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphoglycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r | |
3-phosphoglycerate + NAD+ | allosteric inhibition by L-serine, L-serine regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364, 1 serine binds per subunit | Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.128 | - |
alpha-ketoglutarate | - |
Escherichia coli | |
0.137 | - |
alpha-ketoglutarate | double mutant H344A/N364A | Escherichia coli | |
0.32 | - |
alpha-ketoglutarate | mutant N346A | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli |