Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 100 mM, 68% inhibition, 10 mM, 8% inhibition | Escherichia coli | |
DL-glycerol 3-phosphate | 0.05 mM, 26% inhibition | Escherichia coli | |
glycerol 3-phosphate | 50% inhibition with 0.035 mM, sigmoid inhibition curve, competitive inhibition vs. glycerone phosphate, uncompetitive vs. NADPH | Escherichia coli | |
K2PO4- | 100 mM, 95% inhibition, 10 mM, 27% inhibition | Escherichia coli | |
K2SO4 | 100 mM, 60% inhibition | Escherichia coli | |
KCl | 100 mM, 47% inhibition | Escherichia coli | |
Na2PO4- | 100 mM, 92% inhibition, 10 mM, 27% inhibition | Escherichia coli | |
Na2SO4 | 100 mM, 63% inhibition | Escherichia coli | |
NaCl | 100 mM, 47% inhibition | Escherichia coli | |
NH4Cl | 100 mM, 37% inhibition | Escherichia coli | |
palmitoyl-CoA | 0.0015 mM, 52% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | - |
Escherichia coli | |
0.17 | - |
glycerone phosphate | - |
Escherichia coli | |
0.21 | - |
glycerol-3-phosphate | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD(P)+ | Escherichia coli | - |
glycerone phosphate + NAD(P)H + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
streptomycin, ammonium sulfate, DEAE-Sephadex, Sephadex G-150, DEAE-Sephadex | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4800 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD(P)+ | - |
Escherichia coli | glycerone phosphate + NAD(P)H + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
deamino-NAD+ | 2fold activity compared to NAD+ | Escherichia coli | |
NAD+ | - |
Escherichia coli | |
NADP+ | 2fold activity compared to NAD+ | Escherichia coli |