Cloned (Comment) | Organism |
---|---|
gene lp_3054, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109 | Lactiplantibacillus plantarum WCFS1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 76% inhibition | Lactiplantibacillus plantarum | |
2-mercaptoethanol | inhibits 76% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
Ag+ | inhibits 58% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
AgNO3 | 10 mM, 58% inhibition | Lactiplantibacillus plantarum | |
DMSO | 10 mM, 20% inhibition | Lactiplantibacillus plantarum | |
DMSO | inhibits 20% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
DTT | 10 mM, 84% inhibition | Lactiplantibacillus plantarum | |
DTT | inhibits 84% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
EDTA | 10 mM, 22% inhibition | Lactiplantibacillus plantarum | |
EDTA | inhibits 22% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
SDS | 10 mM, 86% inhibition | Lactiplantibacillus plantarum | |
SDS | inhibits 86% at 10 mM | Lactiplantibacillus plantarum WCFS1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
benzyl alcohol | - |
Lactiplantibacillus plantarum | |
0.23 | - |
benzyl alcohol | pH 5.0, 30°C | Lactiplantibacillus plantarum WCFS1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 10 mM, 1.3fold increase in activity | Lactiplantibacillus plantarum | |
CaCl2 | increases the enzyme activity by 27% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
CdCl2 | 10 mM, 1.3fold increase in activity | Lactiplantibacillus plantarum | |
CdCl2 | increases the enzyme activity by 25% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
KBr | 10 mM, 1.1fold increase in activity | Lactiplantibacillus plantarum | |
KBr | increases the enzyme activity by 11% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
MgCl2 | 10 mM, 1.2fold increase in activity | Lactiplantibacillus plantarum | |
MgCl2 | increases the enzyme activity by 22% at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
additional information | no effects on the enzyme activity by KCl, NaCl, and NiCl2, and by 10 mM urea and Tween-80 | Lactiplantibacillus plantarum WCFS1 | |
Zn | zinc-dependent alcohol dehydrogenase. All of the residues involved in zinc binding are conserved | Lactiplantibacillus plantarum | |
Zn2+ | dependent on, a catalytic zinc atom bound at the active site to the ligands Cys46, Asp49, His67, and Cys174 and a structural zinc atom bound to cysteine residues 97, 100, 103, and 111. All of the residues involved in zinc binding are conserved | Lactiplantibacillus plantarum WCFS1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 | - |
benzaldehyde + NAD(P)H + H+ | - |
? | |
benzyl alcohol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | - |
benzaldehyde + NAD(P)H + H+ | - |
? | |
cinnamyl alcohol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 | - |
cinnamyl aldehyde + NAD(P)H + H+ | - |
? | |
cinnamyl alcohol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | - |
cinnamyl aldehyde + NAD(P)H + H+ | - |
? | |
coniferyl alcohol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 | - |
coniferyl aldehyde + NAD(P)H + H+ | - |
? | |
coniferyl alcohol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | - |
coniferyl aldehyde + NAD(P)H + H+ | - |
? | |
geraniol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 | - |
geranial + NAD(P)H + H+ | - |
? | |
nerol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 | - |
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+ | - |
? | |
phenethyl alcohol + NAD(P)+ | Lactiplantibacillus plantarum WCFS1 | - |
phenylacetaldehyde + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Lactiplantibacillus plantarum WCFS1 | - |
gene lp_3054 | - |
Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | - |
gene lp_3054 | - |
Purification (Comment) | Organism |
---|---|
recombinant lp_3054 from Escherichia coli strain JM109 | Lactiplantibacillus plantarum WCFS1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 | benzaldehyde + NAD(P)H + H+ | - |
? | |
benzyl alcohol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | benzaldehyde + NAD(P)H + H+ | - |
? | |
benzyl alcohol + NAD+ | less active in the presence of NADP+ than in the presence of NAD+ | Lactiplantibacillus plantarum | benzaldehyde + NADH | - |
? | |
benzyl alcohol + NADP+ | less active in the presence of NADP+ than in the presence of NAD+ | Lactiplantibacillus plantarum | benzaldehyde + NADPH | - |
? | |
cinnamyl alcohol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 | cinnamyl aldehyde + NAD(P)H + H+ | - |
? | |
cinnamyl alcohol + NAD(P)+ | 67% activity compared to activity with benzyl alcohol | Lactiplantibacillus plantarum WCFS1 | cinnamyl aldehyde + NAD(P)H + H+ | - |
? | |
cinnamyl alcohol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | cinnamyl aldehyde + NAD(P)H + H+ | - |
? | |
cinnamyl alcohol + NAD(P)+ | 67% activity compared to activity with benzyl alcohol | Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | cinnamyl aldehyde + NAD(P)H + H+ | - |
? | |
cinnamyl alcohol + NAD+ | 67% of the activity with benzyl alcohol | Lactiplantibacillus plantarum | cinnamyl aldehyde + NADH + H+ | - |
? | |
coniferyl alcohol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 | coniferyl aldehyde + NAD(P)H + H+ | - |
? | |
coniferyl alcohol + NAD(P)+ | 59% activity compared to activity with benzyl alcohol | Lactiplantibacillus plantarum WCFS1 | coniferyl aldehyde + NAD(P)H + H+ | - |
? | |
coniferyl alcohol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | coniferyl aldehyde + NAD(P)H + H+ | - |
? | |
coniferyl alcohol + NAD+ | 59% of the activity with benzyl alcohol | Lactiplantibacillus plantarum | coniferyl aldehyde + NADH + H+ | - |
? | |
geraniol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 | geranial + NAD(P)H + H+ | - |
? | |
geraniol + NAD(P)+ | 105% activity compared to activity with benzyl alcohol | Lactiplantibacillus plantarum WCFS1 | geranial + NAD(P)H + H+ | - |
? | |
geraniol + NAD+ | 105% of the activity with benzyl alcohol | Lactiplantibacillus plantarum | (2Z)-3,7-dimethylocta-2,6-dienal + NADH + H+ | - |
? | |
additional information | no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid | Lactiplantibacillus plantarum WCFS1 | ? | - |
? | |
additional information | no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid | Lactiplantibacillus plantarum WCFS1 NCIMB 8826 | ? | - |
? | |
nerol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 | (2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+ | - |
? | |
nerol + NAD(P)+ | 116% activity compared to activity with benzyl alcohol | Lactiplantibacillus plantarum WCFS1 | (2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+ | - |
? | |
nerol + NAD+ | 116% of the activity with benzyl alcohol | Lactiplantibacillus plantarum | (2E)-3,7-dimethylocta-2,6-dienal + NADH + H+ | - |
? | |
phenethyl alcohol + NAD(P)+ | - |
Lactiplantibacillus plantarum WCFS1 | phenylacetaldehyde + NAD(P)H + H+ | - |
? | |
phenethyl alcohol + NAD(P)+ | 73% activity compared to activity with benzyl alcohol | Lactiplantibacillus plantarum WCFS1 | phenylacetaldehyde + NAD(P)H + H+ | - |
? | |
phenethyl alcohol + NAD+ | 73% of the activity with benzyl alcohol | Lactiplantibacillus plantarum | phenylacetaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant enzyme, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
Synonyms | Comment | Organism |
---|---|---|
BADH | - |
Lactiplantibacillus plantarum WCFS1 |
benzyl alcohol dehydrogenase | - |
Lactiplantibacillus plantarum WCFS1 |
More | the enzyme is a member of the family of NAD(P)-dependent long-chain zinc-dependent alcohol dehydrogenases | Lactiplantibacillus plantarum WCFS1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lactiplantibacillus plantarum |
30 | - |
- |
Lactiplantibacillus plantarum WCFS1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 60% of maximal activity, 50°C: about 70% of maximal activity | Lactiplantibacillus plantarum |
25 | 50 | activity range | Lactiplantibacillus plantarum WCFS1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the purified recombinant enzyme remains quite stable | Lactiplantibacillus plantarum WCFS1 |
70 | - |
purified recombinant enzyme, BADH activity significantly decreases to 16% of maximal activity | Lactiplantibacillus plantarum WCFS1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
benzyl alcohol | pH 5.0, 30°C | Lactiplantibacillus plantarum WCFS1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Lactiplantibacillus plantarum |
5 | - |
- |
Lactiplantibacillus plantarum WCFS1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | pH 3.5: about 60% of maximal activity, pH 6.0: about 45% of maximal activity | Lactiplantibacillus plantarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor compared to NADP+, all of the residues involved in cofactor binding are conserved. Residues Thr178 and Val203 interact with the nicotinamide ring, polypeptide backbone nitrogen atoms of residues Gly201 and Gly202 interact with an oxygen of the phosphate proximal to the nicotinamide group, and His47 binds the diphosphate of the NAD+ coenzyme. An Asp residue determines the specificity for NAD+ | Lactiplantibacillus plantarum WCFS1 | |
NADP+ | preferred cofactor is NAD+, all of the residues involved in cofactor binding are conserved. An Asp residue determines the specificity for NAD+ | Lactiplantibacillus plantarum WCFS1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lactiplantibacillus plantarum WCFS1 | sequence calculation | - |
5.04 |