General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Pseudomonas putida |
benzyl alcohol stabilizes slightly | Pseudomonas putida |
dithiothreitol stabilizes | Pseudomonas putida |
MgCl2 stabilizes slightly | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | above 2.5 mM | Pseudomonas putida | |
iodoacetamide | slight | Pseudomonas putida | |
N-ethylmaleimide | - |
Pseudomonas putida | |
NADH | noncompetitive | Pseudomonas putida | |
p-chloromercuribenzoate | - |
Pseudomonas putida | |
SH-blocking agents | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0257 | - |
benzaldehyde | - |
Pseudomonas putida | |
0.047 | - |
NADH | - |
Pseudomonas putida | |
0.0476 | - |
NAD+ | - |
Pseudomonas putida | |
0.2194 | - |
benzyl alcohol | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KBr | - |
Pseudomonas putida | |
KCl | activity nearly doubled | Pseudomonas putida | |
KNO3 | - |
Pseudomonas putida | |
MgCl2 | slight activation | Pseudomonas putida | |
NaCl | - |
Pseudomonas putida | |
NH4Cl | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Pseudomonas putida |
90000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | Pseudomonas putida | - |
benzaldehyde + NADH | - |
r | |
benzyl alcohol + NAD+ | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
benzaldehyde + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme encoded by plasmid TOL | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
230.3 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-70°C, enzyme dialyzed against buffer containing 40% glycerol | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 2-aminobenzaldehyde + NADH | - |
r | |
2-hydroxymethylthiophene + NAD+ | - |
Pseudomonas putida | 2-formylthiophene + NADH | - |
r | |
2-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 2-methylbenzaldehyde + NADH + H+ | - |
r | |
2-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 2-methylbenzaldehyde + NADH + H+ | - |
r | |
3,4-dimethylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3,4-dimethylbenzaldehyde + NADH + H+ | - |
r | |
3-aminobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-aminobenzaldehyde + NADH | - |
r | |
3-chlorobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-chlorobenzaldehyde + NADH | - |
r | |
3-hydroxybenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-hydroxybenzaldehyde + NADH | - |
r | |
3-methoxybenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-methoxybenzaldehyde + NADH | - |
r | |
3-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-methylbenzaldehyde + NADH + H+ | - |
r | |
3-nitrobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-nitrobenzaldehyde + NADH | - |
r | |
4-aminobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 4-aminobenzaldehyde + NADH | - |
r | |
4-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 4-methylbenzaldehyde + NADH + H+ | - |
r | |
benzyl alcohol + NAD+ | - |
Pseudomonas putida | benzaldehyde + NADH | - |
r | |
benzyl alcohol + NAD+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | benzaldehyde + NADH | - |
r | |
furfuryl alcohol + NAD+ | - |
Pseudomonas putida | furfural + NADH | - |
r | |
furfuryl alcohol + NAD+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | furfural + NADH | - |
r | |
additional information | not: 4-nitrophenyl acetate | Pseudomonas putida | ? | - |
? | |
additional information | a group of enzymes with broad specificity towards alcohols with an aromatic or cyclohex-1-ene ring, but with low or no activity towards short chain aliphatic alcohols | Pseudomonas putida | ? | - |
? | |
additional information | not: 4-nitrophenyl acetate | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | ? | - |
? | |
additional information | a group of enzymes with broad specificity towards alcohols with an aromatic or cyclohex-1-ene ring, but with low or no activity towards short chain aliphatic alcohols | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
after 21 h no loss of activity | Pseudomonas putida |
56 | - |
after 10 min complete loss of activity | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
228 | - |
benzyl alcohol | - |
Pseudomonas putida | |
622 | - |
benzaldehyde | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | pH 7.5: about 15% of activity maximum, pH 10.5: about 20% of activity maximum | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | contains no prosthetic group such as FAD or FMN | Pseudomonas putida | |
NAD+ | enzyme transfers hydride to the pro-R side of the prochiral C4 to the pyridine ring of NAD+ | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
NADH | - |
Pseudomonas putida |