Cloned (Comment) | Organism |
---|---|
gene xyl2, overexpression in Gluconobacter strain PXPG, coexpression with the glucose dehydrogenase gene from Bacillus subtilis | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Gluconobacter oxydans | - |
D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | Gluconobacter oxydans NH-10 | - |
D-xylulose + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q8GR61 | - |
- |
Gluconobacter oxydans NH-10 | Q8GR61 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme XDH depends exclusively on NAD+/NADH as cofactors | Gluconobacter oxydans | ? | - |
? | |
additional information | enzyme XDH depends exclusively on NAD+/NADH as cofactors | Gluconobacter oxydans NH-10 | ? | - |
? | |
xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Gluconobacter oxydans NH-10 | D-xylulose + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
XDH | - |
Gluconobacter oxydans |
XYL2 | - |
Gluconobacter oxydans |
xylitol dehydrogenase | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Gluconobacter oxydans | |
NADH | - |
Gluconobacter oxydans |
Organism | Comment | Expression |
---|---|---|
Gluconobacter oxydans | the engineered Gluconobacter oxydans strain PXPG is constructed to coexpress the endogenous XDH gene and the cofactor regeneration glucose dehydrogenase gene from Bacillus subtilis. Activities for both enzymes are more than twofold higher in the Gluconobacter oxydans PXPG than in the wild-type strain. Increasing the XDH activity and the cofactor NADH supply improves the xylitol productivity | additional information |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity directly limiting the overall conversion process of D-xylose fermentation to ethanol by Gluconobacter oxydans | Gluconobacter oxydans |