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Literature summary for 1.1.1.9 extracted from

  • Khattab, S.M.; Saimura, M.; Kodaki, T.
    Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP+-dependent xylitol dehydrogenase (2013), J. Biotechnol., 165, 153-156.
    View publication on PubMed
Search for more literature for 1.1.1.9

Protein Variants

Protein Variants Comment Organism
additional information enzyme XDH is changed from NAD+-dependent to NADP+-dependent, xylitol accumulation is reduced and ethanol production improved using protein engineering for reversing the dependency of XDH from NAD+ to NADP+. Construction of a set of recombinant Saccharomyces cerevisiae carrying a mutated strictly NADPH-dependent XR and NADP+-dependent XDH genes with overexpression of endogenous xylulokinase (XK), effects of complete NADPH/NADP+ recycling on ethanol fermentation and xylitol accumulation, overview. The mutated strains demonstrate 0% and 10% improvement in ethanol production, and reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain Scheffersomyces stipitis

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis P22144 i.e Pichia stipitis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
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 Scheffersomyces stipitis L-xylulose + NADH + H+
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Synonyms

Synonyms Comment Organism
NAD+-dependent xylitol dehydrogenase
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 Scheffersomyces stipitis
XDH
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 Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Scheffersomyces stipitis

General Information

General Information Comment Organism
metabolism the redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH) is thought to be an important factor in effective xylose fermentation Scheffersomyces stipitis
physiological function strictly NADPH-dependent XR with mutated strict NADP+-dependent XDH are more effective in increasing bioethanol production and decreasing xylitol accumulation than the NAD+-dependent wild-type XDH, overview Scheffersomyces stipitis