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Literature summary for 1.1.1.9 extracted from

  • Hickman, J.; Ashwell, G.
    A sensitive and stereospecific enzymatic assay for xylulose (1959), J. Biol. Chem., 234, 758-761.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine of partially purified preparation Cavia porcellus

General Stability

General Stability Organism
crude water extract from liver acetone-powder unstable Cavia porcellus
freezing/thawing inactivates, 8 cycles lead to 89% loss of activity and 11 cycles to complete inactivation Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
D-xylulose high concentration Cavia porcellus
additional information no inhibition by EDTA or iodoacetate Cavia porcellus
PCMB complete inhibition Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
D-xylulose
-
Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Cavia porcellus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no evidence for metal requirement Cavia porcellus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone stable to precipitation with 50% v/v Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cavia porcellus

Storage Stability

Storage Stability Organism
-10°C, acetone precipitate stable for weeks Cavia porcellus
0°C, partially purified stable for weeks Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribitol + NAD+
-
Cavia porcellus D-ribulose + NADH
-
?
D-sorbitol + NAD+
-
Cavia porcellus D-fructose + NADH + H+
-
?
D-xylulose + NADH + H+
-
Cavia porcellus xylitol + NAD+
-
r
L-arabitol + NAD+
-
Cavia porcellus ?
-
?
L-erythrulose + NADH reduction at the same rate as D-xylulose Cavia porcellus erythritol + NAD+
-
?
L-iditol + NAD+
-
Cavia porcellus ?
-
?
additional information no reduction of D-ribose and D-galacturonic acid Cavia porcellus ?
-
?
additional information no oxidation of threitol, xylitol, sorbitol, D-iditol Cavia porcellus ?
-
?
additional information no reduction of D-ribulose Cavia porcellus ?
-
?
additional information no reduction of D/L-xylose Cavia porcellus ?
-
?
xylitol + NAD+
-
Cavia porcellus D-xylulose + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
substrate reduction Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
4 8 about 75% of maximal reduction activity at pH 4.0 and 8.0 Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Cavia porcellus
additional information no activity with NADP+ Cavia porcellus
additional information at high concentrations of xylitol 1.5% as effective as NAD+ Cavia porcellus
NAD+
-
Cavia porcellus
NADH
-
Cavia porcellus