Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | of partially purified preparation | Cavia porcellus |
General Stability | Organism |
---|---|
crude water extract from liver acetone-powder unstable | Cavia porcellus |
freezing/thawing inactivates, 8 cycles lead to 89% loss of activity and 11 cycles to complete inactivation | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-xylulose | high concentration | Cavia porcellus | |
additional information | no inhibition by EDTA or iodoacetate | Cavia porcellus | |
PCMB | complete inhibition | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
D-xylulose | - |
Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Cavia porcellus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no evidence for metal requirement | Cavia porcellus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | stable to precipitation with 50% v/v | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
-10°C, acetone precipitate stable for weeks | Cavia porcellus |
0°C, partially purified stable for weeks | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribitol + NAD+ | - |
Cavia porcellus | D-ribulose + NADH | - |
? | |
D-sorbitol + NAD+ | - |
Cavia porcellus | D-fructose + NADH + H+ | - |
? | |
D-xylulose + NADH + H+ | - |
Cavia porcellus | xylitol + NAD+ | - |
r | |
L-arabitol + NAD+ | - |
Cavia porcellus | ? | - |
? | |
L-erythrulose + NADH | reduction at the same rate as D-xylulose | Cavia porcellus | erythritol + NAD+ | - |
? | |
L-iditol + NAD+ | - |
Cavia porcellus | ? | - |
? | |
additional information | no reduction of D-ribose and D-galacturonic acid | Cavia porcellus | ? | - |
? | |
additional information | no oxidation of threitol, xylitol, sorbitol, D-iditol | Cavia porcellus | ? | - |
? | |
additional information | no reduction of D-ribulose | Cavia porcellus | ? | - |
? | |
additional information | no reduction of D/L-xylose | Cavia porcellus | ? | - |
? | |
xylitol + NAD+ | - |
Cavia porcellus | D-xylulose + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
substrate reduction | Cavia porcellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | about 75% of maximal reduction activity at pH 4.0 and 8.0 | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Cavia porcellus | |
additional information | no activity with NADP+ | Cavia porcellus | |
additional information | at high concentrations of xylitol 1.5% as effective as NAD+ | Cavia porcellus | |
NAD+ | - |
Cavia porcellus | |
NADH | - |
Cavia porcellus |