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Literature summary for 1.1.1.86 extracted from

  • Cahn, J.K.; Brinkmann-Chen, S.; Spatzal, T.; Wiig, J.A.; Buller, A.R.; Einsle, O.; Hu, Y.; Ribbe, M.W.; Arnold, F.H.
    Cofactor specificity motifs and the induced fit mechanism in Class I ketol-acid reductoisomerases (2015), Biochem. J., 468, 475-484.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 5.0, 20% (w/v) w/v polyethylene glycol 1500 uncultured archaeon
hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 6.0, 22% (w/v) polyethylene glycol monomethylether 5000 Ignisphaera aggregans
hanging drop vapor diffusion method, using 0.25 M NaCl, 28% (w/v) polyethylene glycol 3350 and 0.1 M bis-Tris, pH 5.5 Azotobacter vinelandii
hanging drop vapor diffusion method, using 1 M sodium potassium tartrate, 200 mM sodium chloride, 100 mM imidazole pH 8.0 Alicyclobacillus acidocaldarius subsp. acidocaldarius
in complex with NADPH and the transition state analogue N-isopropyloxamate and apo-form. The enzyme has a seven-residue specificity loop Ignisphaera aggregans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Ignisphaera aggregans
Mg2+ required Azotobacter vinelandii
Mg2+ required Alicyclobacillus acidocaldarius subsp. acidocaldarius
Mg2+ required uncultured archaeon

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-acetolactate + NADH + H+ Ignisphaera aggregans
-
(R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+ Azotobacter vinelandii
-
(R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+ Alicyclobacillus acidocaldarius subsp. acidocaldarius
-
(R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+ uncultured archaeon
-
(R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+ Ignisphaera aggregans DSM 17230
-
(R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+ Azotobacter vinelandii ATCC BAA-1303
-
(R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADPH + H+ Ignisphaera aggregans
-
(R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+ Azotobacter vinelandii
-
(R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+ Alicyclobacillus acidocaldarius subsp. acidocaldarius
-
(R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+ uncultured archaeon
-
(R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+ Ignisphaera aggregans DSM 17230
-
(R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+ Azotobacter vinelandii ATCC BAA-1303
-
(R)-2,3-dihydroxyisovalerate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius subsp. acidocaldarius C8WR67
-
-
Azotobacter vinelandii C1DFH7
-
-
Azotobacter vinelandii ATCC BAA-1303 C1DFH7
-
-
Ignisphaera aggregans E0SRA9
-
-
Ignisphaera aggregans DSM 17230 E0SRA9
-
-
uncultured archaeon Q64BR7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-acetolactate + NADH + H+
-
Ignisphaera aggregans (R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+
-
Azotobacter vinelandii (R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius (R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+
-
uncultured archaeon (R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+
-
Ignisphaera aggregans DSM 17230 (R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADH + H+
-
Azotobacter vinelandii ATCC BAA-1303 (R)-2,3-dihydroxyisovalerate + NAD+
-
r
(S)-2-acetolactate + NADPH + H+
-
Ignisphaera aggregans (R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+
-
Azotobacter vinelandii (R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius (R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+
-
uncultured archaeon (R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+
-
Ignisphaera aggregans DSM 17230 (R)-2,3-dihydroxyisovalerate + NADP+
-
r
(S)-2-acetolactate + NADPH + H+
-
Azotobacter vinelandii ATCC BAA-1303 (R)-2,3-dihydroxyisovalerate + NADP+
-
r

Synonyms

Synonyms Comment Organism
acetohydroxyacid isomeroreductase
-
Ignisphaera aggregans
acetohydroxyacid isomeroreductase
-
Azotobacter vinelandii
acetohydroxyacid isomeroreductase
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
acetohydroxyacid isomeroreductase
-
uncultured archaeon
AHAIR
-
Ignisphaera aggregans
AHAIR
-
Azotobacter vinelandii
AHAIR
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
AHAIR
-
uncultured archaeon
AHIR
-
Ignisphaera aggregans
AHIR
-
Azotobacter vinelandii
AHIR
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
AHIR
-
uncultured archaeon
ilvC
-
Ignisphaera aggregans
KARI
-
Ignisphaera aggregans
KARI
-
Azotobacter vinelandii
KARI
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
KARI
-
uncultured archaeon

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
95
-
half-maximal residual activity at 95°C Ignisphaera aggregans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
the enzyme is highly thermostable with half-maximal residual activity at 95°C Ignisphaera aggregans

Cofactor

Cofactor Comment Organism Structure
NADH
-
Ignisphaera aggregans
NADH
-
Azotobacter vinelandii
NADH
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
NADH
-
uncultured archaeon
NADPH
-
Ignisphaera aggregans
NADPH
-
Azotobacter vinelandii
NADPH
-
Alicyclobacillus acidocaldarius subsp. acidocaldarius
NADPH
-
uncultured archaeon