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Literature summary for 1.1.1.86 extracted from

  • Dumas, R.; Cornillon-Bertrand, C.; Guigue-Talet, P.; Genix, P.; Douce, R.; Job, D.
    Interactions of plant acetohydroxy acid isomeroreductase with reaction intermediate analogues: correlation of the slow, competitive, inhibition kinetics of enzyme activity and herbicidal effects (1994), Biochem. J., 301, 813-820.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-dimethylphosphinoyl-2-hydroxyacetic acid nearly irreversible; reversible, strong competitive inhibition Ipomoea purpurea
2-dimethylphosphinoyl-2-hydroxyacetic acid nearly irreversible; reversible, strong competitive inhibition Solanum nigrum
2-dimethylphosphinoyl-2-hydroxyacetic acid nearly irreversible; reversible, strong competitive inhibition Spinacia oleracea
N-Hydroxy-N-isopropyloxamate nearly irreversible, competitive; nearly irreversible, poor herbicidal action Ipomoea purpurea
N-Hydroxy-N-isopropyloxamate nearly irreversible, competitive; nearly irreversible, poor herbicidal action Solanum nigrum
N-Hydroxy-N-isopropyloxamate nearly irreversible, competitive; nearly irreversible, poor herbicidal action Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Spinacia oleracea 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Spinacia oleracea
Mg2+ required Ipomoea purpurea
Mg2+ required Solanum nigrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-acetolactate + NADPH Spinacia oleracea enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH Ipomoea purpurea enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH Solanum nigrum enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Ipomoea purpurea
-
morning glory
-
Solanum nigrum
-
black nightshade
-
Spinacia oleracea
-
overexpressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Spinacia oleracea 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Ipomoea purpurea 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Solanum nigrum 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
NADPH + 2-acetolactate
-
Spinacia oleracea NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
?
NADPH + 2-acetolactate
-
Ipomoea purpurea NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
?
NADPH + 2-acetolactate
-
Solanum nigrum NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Spinacia oleracea
NADPH
-
Ipomoea purpurea
NADPH
-
Solanum nigrum