Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | competitive with NADPH | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
NADPH | pH 8, 30°C, isoenzyme 1 | Spinacia oleracea | |
0.025 | - |
2-acetolactate | pH 8, 30°C, isoenzyme 1 | Spinacia oleracea | |
0.037 | - |
2-aceto-2-hydroxybutyrate | pH 8, 30°C, isoenzyme 1 | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
4 * 59000, SDS-PAGE | Spinacia oleracea |
205000 | - |
SDS-PAGE, isoenzyme 3 | Spinacia oleracea |
220000 | - |
SDS-PAGE, isoenzyme 2 | Spinacia oleracea |
235000 | - |
SDS-PAGE, isoenzyme 1 | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | Spinacia oleracea | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach, multiple enzyme forms: iso 1, 2 and 3 | - |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.79 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Spinacia oleracea | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
NADPH + 2-aceto-2-hydroxybutyrate | - |
Spinacia oleracea | NADP+ + ? | - |
? | |
NADPH + 2-acetolactate | - |
Spinacia oleracea | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 59000, SDS-PAGE | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | reaction with 2-acetolactate, isoenzyme 1 | Spinacia oleracea |
8 | 8.5 | reaction with 2-aceto-2-hydroxybutyrate | Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 35% of maximal activity, pH 8.5: about 90% of maximal activity, reaction with 2-acetolactate, isoenzyme 1 | Spinacia oleracea |
7 | 8.5 | pH 7.0: about 25% of maximal activity, pH 8.0-8.5: optimum, isoenzyme 1 | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Spinacia oleracea |