Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)Codon-Plus-RIL cells | Sulfolobus acidocaldarius |
Crystallization (Comment) | Organism |
---|---|
in complex with (2R,3S)-3-isopropylmalate and Mg2+, hanging drop vapor diffusion method, using 2.0 M (NH4)2SO4, 200 mM NaCl, and 100 mM sodium cacodylate pH 6.5 | Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
(2R,3S)-3-isopropylmalate | at pH 8.0 and 60°C | Sulfolobus acidocaldarius | |
0.12 | - |
NAD+ | at pH 8.0 and 60°C | Sulfolobus acidocaldarius | |
5.9 | - |
homoisocitrate | at pH 8.0 and 60°C | Sulfolobus acidocaldarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
4 * 36500, SDS-PAGE | Sulfolobus acidocaldarius |
130000 | - |
gel filtration | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | Sulfolobus acidocaldarius | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | Sulfolobus acidocaldarius MW001 | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q4JB37 | - |
- |
Sulfolobus acidocaldarius MW001 | Q4JB37 | - |
- |
Purification (Comment) | Organism |
---|---|
DE52 column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Sulfolobus acidocaldarius | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Sulfolobus acidocaldarius MW001 | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
homoisocitrate + NAD+ | - |
Sulfolobus acidocaldarius | ? + NADH + H+ | - |
? | |
homoisocitrate + NAD+ | - |
Sulfolobus acidocaldarius MW001 | ? + NADH + H+ | - |
? | |
additional information | the enzyme does not show detectable activity for isocitrate using NAD+ as a coenzyme, and is inactive with NADP+ | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | the enzyme does not show detectable activity for isocitrate using NAD+ as a coenzyme, and is inactive with NADP+ | Sulfolobus acidocaldarius MW001 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36500, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
IPMDH | - |
Sulfolobus acidocaldarius |
Saci_0600 | - |
Sulfolobus acidocaldarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
homoisocitrate | at pH 8.0 and 60°C | Sulfolobus acidocaldarius | |
8.5 | - |
(2R,3S)-3-isopropylmalate | at pH 8.0 and 60°C | Sulfolobus acidocaldarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Sulfolobus acidocaldarius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
440 | - |
homoisocitrate | at pH 8.0 and 60°C | Sulfolobus acidocaldarius | |
1700 | - |
(2R,3S)-3-isopropylmalate | at pH 8.0 and 60°C | Sulfolobus acidocaldarius |