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Literature summary for 1.1.1.85 extracted from

  • Takahashi, K.; Nakanishi, F.; Tomita, T.; Akiyama, N.; Lassak, K.; Albers, S.V.; Kuzuyama, T.; Nishiyama, M.
    Characterization of two ?-decarboxylating dehydrogenases from Sulfolobus acidocaldarius (2016), Extremophiles, 20, 843-853.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)Codon-Plus-RIL cells Sulfolobus acidocaldarius

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with (2R,3S)-3-isopropylmalate and Mg2+, hanging drop vapor diffusion method, using 2.0 M (NH4)2SO4, 200 mM NaCl, and 100 mM sodium cacodylate pH 6.5 Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
(2R,3S)-3-isopropylmalate at pH 8.0 and 60°C Sulfolobus acidocaldarius
0.12
-
NAD+ at pH 8.0 and 60°C Sulfolobus acidocaldarius
5.9
-
homoisocitrate at pH 8.0 and 60°C Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
4 * 36500, SDS-PAGE Sulfolobus acidocaldarius
130000
-
gel filtration Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+ Sulfolobus acidocaldarius
-
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?
(2R,3S)-3-isopropylmalate + NAD+ Sulfolobus acidocaldarius MW001
-
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4JB37
-
-
Sulfolobus acidocaldarius MW001 Q4JB37
-
-

Purification (Commentary)

Purification (Comment) Organism
DE52 column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Sulfolobus acidocaldarius (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Sulfolobus acidocaldarius MW001 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?
homoisocitrate + NAD+
-
Sulfolobus acidocaldarius ? + NADH + H+
-
?
homoisocitrate + NAD+
-
Sulfolobus acidocaldarius MW001 ? + NADH + H+
-
?
additional information the enzyme does not show detectable activity for isocitrate using NAD+ as a coenzyme, and is inactive with NADP+ Sulfolobus acidocaldarius ?
-
?
additional information the enzyme does not show detectable activity for isocitrate using NAD+ as a coenzyme, and is inactive with NADP+ Sulfolobus acidocaldarius MW001 ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 36500, SDS-PAGE Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
IPMDH
-
Sulfolobus acidocaldarius
Saci_0600
-
Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
homoisocitrate at pH 8.0 and 60°C Sulfolobus acidocaldarius
8.5
-
(2R,3S)-3-isopropylmalate at pH 8.0 and 60°C Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
440
-
homoisocitrate at pH 8.0 and 60°C Sulfolobus acidocaldarius
1700
-
(2R,3S)-3-isopropylmalate at pH 8.0 and 60°C Sulfolobus acidocaldarius