Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli HB101 cells | Shewanella benthica |
expressed in Escherichia coli HB101 cells | Shewanella oneidensis |
Protein Variants | Comment | Organism |
---|---|---|
A266S | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Shewanella benthica |
A268I | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Shewanella oneidensis |
I268A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Shewanella benthica |
L106M | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Shewanella oneidensis |
M106L | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Shewanella benthica |
S266A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Shewanella oneidensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0122 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme L106M, at pH 7.6 and 25°C | Shewanella oneidensis | |
0.0122 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at pH 7.6 and 25°C | Shewanella benthica | |
0.0141 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at pH 7.6 and 25°C | Shewanella oneidensis | |
0.0226 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme S266A, at pH 7.6 and 25°C | Shewanella oneidensis | |
0.0239 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme M106L, at pH 7.6 and 25°C | Shewanella benthica | |
0.0344 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A266S, at pH 7.6 and 25°C | Shewanella benthica | |
0.0345 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A268I, at pH 7.6 and 25°C | Shewanella oneidensis | |
0.0356 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme I268A, at pH 7.6 and 25°C | Shewanella benthica | |
0.0708 | - |
NAD+ | mutant enzyme L106M, at pH 7.6 and 25°C | Shewanella oneidensis | |
0.0756 | - |
NAD+ | mutant enzyme S266A, at pH 7.6 and 25°C | Shewanella oneidensis | |
0.079 | - |
NAD+ | mutant enzyme M106L, at pH 7.6 and 25°C | Shewanella benthica | |
0.0814 | - |
NAD+ | wild type enzyme, at pH 7.6 and 25°C | Shewanella oneidensis | |
0.1058 | - |
NAD+ | wild type enzyme, at pH 7.6 and 25°C | Shewanella benthica | |
0.1707 | - |
NAD+ | mutant enzyme I268A, at pH 7.6 and 25°C | Shewanella benthica | |
0.2379 | - |
NAD+ | mutant enzyme A266S, at pH 7.6 and 25°C | Shewanella benthica | |
0.2785 | - |
NAD+ | mutant enzyme A268I, at pH 7.6 and 25°C | Shewanella oneidensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | Shewanella benthica | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | Shewanella oneidensis | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | Shewanella benthica DB21MT-2 | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | Shewanella oneidensis MR-1 / ATCC 700550 | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella benthica | - |
- |
- |
Shewanella benthica DB21MT-2 | - |
- |
- |
Shewanella oneidensis | Q8E9N3 | - |
- |
Shewanella oneidensis MR-1 / ATCC 700550 | Q8E9N3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose 6 column chromatography | Shewanella benthica |
Ni Sepharose 6 column chromatography | Shewanella oneidensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Shewanella benthica | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Shewanella oneidensis | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Shewanella benthica DB21MT-2 | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Shewanella oneidensis MR-1 / ATCC 700550 | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPMDH | - |
Shewanella benthica |
IPMDH | - |
Shewanella oneidensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | NAD+ | mutant enzyme A266S, at pH 7.6 and 25°C | Shewanella benthica | |
9.6 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme I268A, at pH 7.6 and 25°C | Shewanella benthica | |
10.3 | - |
NAD+ | mutant enzyme I268A, at pH 7.6 and 25°C | Shewanella benthica | |
14.8 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme M106L, at pH 7.6 and 25°C | Shewanella benthica | |
15.2 | - |
NAD+ | mutant enzyme M106L, at pH 7.6 and 25°C | Shewanella benthica | |
15.6 | - |
NAD+ | mutant enzyme S266A, at pH 7.6 and 25°C | Shewanella oneidensis | |
16.1 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A268I, at pH 7.6 and 25°C | Shewanella oneidensis | |
16.4 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at pH 7.6 and 25°C | Shewanella benthica | |
16.7 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme S266A, at pH 7.6 and 25°C | Shewanella oneidensis | |
17.1 | - |
NAD+ | wild type enzyme, at pH 7.6 and 25°C | Shewanella oneidensis | |
17.4 | - |
NAD+ | mutant enzyme A268I, at pH 7.6 and 25°C | Shewanella oneidensis | |
17.4 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at pH 7.6 and 25°C | Shewanella oneidensis | |
18.7 | - |
NAD+ | wild type enzyme, at pH 7.6 and 25°C | Shewanella benthica | |
20.4 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A266S, at pH 7.6 and 25°C | Shewanella benthica | |
48.1 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme L106M, at pH 7.6 and 25°C | Shewanella oneidensis | |
50.5 | - |
NAD+ | mutant enzyme L106M, at pH 7.6 and 25°C | Shewanella oneidensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Shewanella benthica | |
NAD+ | - |
Shewanella oneidensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
NAD+ | mutant enzyme I268A, at pH 7.6 and 25°C | Shewanella benthica | |
100 | - |
NAD+ | mutant enzyme A266S, at pH 7.6 and 25°C | Shewanella benthica | |
180 | - |
NAD+ | wild type enzyme, at pH 7.6 and 25°C | Shewanella benthica | |
190 | - |
NAD+ | mutant enzyme M106L, at pH 7.6 and 25°C | Shewanella benthica | |
270 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme I268A, at pH 7.6 and 25°C | Shewanella benthica | |
590 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme A266S, at pH 7.6 and 25°C | Shewanella benthica | |
620 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme M106L, at pH 7.6 and 25°C | Shewanella benthica | |
1350 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at pH 7.6 and 25°C | Shewanella benthica |