Protein Variants | Comment | Organism |
---|---|---|
D217A | the mutant retains 1.1% of the catalytic activity of the wild type enzyme | Thermus thermophilus |
D241A | the mutant shows a drastic decrease in the kcat value (0.06% compared to that of the wild type enzyme) | Thermus thermophilus |
D245A | the mutant retains 10.5% of the catalytic activity of the wild type enzyme | Thermus thermophilus |
E270A | the mutation leads to a significant increase in the Km value for NAD+ compared to the wild type enzyme | Thermus thermophilus |
K185A | the mutant shows a drastic decrease in the kcat value (0.06% compared to that of the wild type enzyme) | Thermus thermophilus |
N102A | the mutant retains 13% of the catalytic activity of the wild type enzyme | Thermus thermophilus |
Y139A | the mutant retains 2.9% of the catalytic activity of the wild type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme D217A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.014 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme N102A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.016 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at pH 7.6 and 20°C | Thermus thermophilus | |
0.02 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme D245A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.032 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme E270A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.04 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme Y139A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.06 | - |
NAD+ | mutant enzyme Y139A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.064 | - |
NAD+ | mutant enzyme K185A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.145 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme K185A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.255 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme D241A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.26 | - |
NAD+ | mutant enzyme N102A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.29 | - |
NAD+ | wild type enzyme, at pH 7.6 and 20°C | Thermus thermophilus | |
0.305 | - |
NAD+ | mutant enzyme D217A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.32 | - |
NAD+ | mutant enzyme D241A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.375 | - |
NAD+ | mutant enzyme D245A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.66 | - |
NAD+ | mutant enzyme E270A, at pH 7.6 and 20°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | Thermus thermophilus | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SIY4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPMDH | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme D241A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.0025 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme K185A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.033 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme E270A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.045 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme D217A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.108 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme Y139A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.433 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme D245A, at pH 7.6 and 20°C | Thermus thermophilus | |
0.55 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme N102A, at pH 7.6 and 20°C | Thermus thermophilus | |
40 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, at pH 7.6 and 20°C | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |