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Literature summary for 1.1.1.85 extracted from

  • He, Y.; Galant, A.; Pang, Q.; Strul, J.; Balogun, S.; Jez, J.; Chen, S.
    Structural and functional evolution of isopropylmalate dehydrogenases in the leucine and glucosinolate pathways of Arabidopsis thaliana (2011), J. Biol. Chem., 286, 28794-28801.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.25 A resolution. Modeling of 3-isopropylmalate binding in the isoform IPMDH2 active site. Results suggest that substitution of one active site residue may lead to altered substrate specificity and metabolic function Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
F137L mutation to corresponding residue of isoform IMPDH2, reduces activity toward 3-(2'-methylthio)-ethylmalate by 200fold, but enhances catalytic efficiency with 3-isopropylmalate to levels observed with isoform IPMDH2 Arabidopsis thaliana
L133F mutation to corresponding residue of isoform IMPDH1, enhances catalytic efficiency with 3-(2'-methylthio)ethylmalate about100fold and reduces activity for 3-isopropylmalate Arabidopsis thaliana
L134F mutation to corresponding residue of isoform IMPDH1, reduces activity toward 3-(2'-methylthio)-ethylmalate, but enhances catalytic efficiency with 3-isopropylmalate Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0092
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.0109
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.011
-
(2R,3S)-3-isopropylmalate mutant F137L, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.025
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.028
-
(2R,3S)-3-isopropylmalate mutant L134F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.0303
-
(2R,3S)-3-isopropylmalate mutant L133F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.045
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.077
-
3-(2'-methylthio)ethylmalate mutant L133F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.103
-
3-(2'-methylthio)ethylmalate mutant L134F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.323
-
3-(2'-methylthio)ethylmalate mutant F137L, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.436
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.502
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P93832 isoform IPMDH2
-
Arabidopsis thaliana Q9FMT1 isoform IPMDH3
-
Arabidopsis thaliana Q9SA14 isoform IPMDH1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Arabidopsis thaliana (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?
3-(2'-methylthio)ethylmalate + NAD+
-
Arabidopsis thaliana ? + NADH + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.0167
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.033
-
3-(2'-methylthio)ethylmalate mutant F137L, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.27
-
3-(2'-methylthio)ethylmalate mutant L134F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.367
-
3-(2'-methylthio)ethylmalate mutant L133F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.62
-
(2R,3S)-3-isopropylmalate mutant L133F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.622
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.73
-
(2R,3S)-3-isopropylmalate mutant L134F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.85
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
3.83
-
(2R,3S)-3-isopropylmalate mutant F137L, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
6.22
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
9.05
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.038
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.1
-
3-(2'-methylthio)ethylmalate mutant F137L, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.33
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
2.59
-
3-(2'-methylthio)ethylmalate mutant L134F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
4.76
-
3-(2'-methylthio)ethylmalate mutant L133F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
18.77
-
3-(2'-methylthio)ethylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
20.3
-
(2R,3S)-3-isopropylmalate mutant L133F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
24.47
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
25.7
-
(2R,3S)-3-isopropylmalate mutant L134F, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
336.3
-
(2R,3S)-3-isopropylmalate mutant F137L, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
570
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
983.7
-
(2R,3S)-3-isopropylmalate wild-type, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana