Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.85 extracted from

  • Sasaki, M.; Uno, M.; Akanuma, S.; Yamagishi, A.
    Random mutagenesis improves the low-temperature activity of the tetrameric 3-isopropylmalate dehydrogenase from the hyperthermophile Sulfolobus tokodaii (2008), Protein Eng. Des. Sel., 21, 721-727.
    View publication on PubMed
Search for more literature for 1.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
gene leuB, expression of wild-type and mutant enzymes in Escherichia coli strain MA153 Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
F145S random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii
F3S random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii
F3S/L288P random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme. The MA153 strain containing a doubly mutated leuB gene grows more rapidly than does Escherichia coli MA153 containing the wild-type leuB gene in leucine-free M9 minimal liquid medium Sulfurisphaera tokodaii
F3S/S232G random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme. The MA153 strain containing a doubly mutated leuB gene grows more rapidly than does Escherichia coli MA153 containing the wild-type leuB gene in leucine-free M9 minimal liquid medium Sulfurisphaera tokodaii
I68L/I142T/K335E random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii
L288P random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii
L328S random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii
L336P random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii
R284G random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii
Y309H random mutagenesis, the mutation alters the Km values for NAD+ and/or the kcat value, the mutant shows reduced thermostability compared to the wild-type enzyme Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics of recombinant wild-type and mutant enzymes at different temperatures, overview Sulfurisphaera tokodaii
0.0039
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant F3S/S232G Sulfurisphaera tokodaii
0.0044
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant L288P Sulfurisphaera tokodaii
0.0055
-
 3-isopropylmalate pH 7.5, 70°C, recombinant wild-type enzyme Sulfurisphaera tokodaii
0.0067
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant F3S/L288P Sulfurisphaera tokodaii
0.0076
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant F3S Sulfurisphaera tokodaii
0.008
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant R284G Sulfurisphaera tokodaii
0.22
-
 NAD+ pH 7.5, 70°C, recombinant mutant F3S/S232G Sulfurisphaera tokodaii
0.25
-
 NAD+ pH 7.5, 70°C, recombinant mutant R284G Sulfurisphaera tokodaii
0.37
-
 NAD+ pH 7.5, 70°C, recombinant mutant L288P Sulfurisphaera tokodaii
0.39
-
 NAD+ pH 7.5, 70°C, recombinant wild-type enzyme and mutant F3S Sulfurisphaera tokodaii
0.4
-
 NAD+ pH 7.5, 70°C, recombinant mutant F3S/L288P Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+ Sulfurisphaera tokodaii the enzyme is enzyme involved in leucine biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii P50455 gene leuB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain MA153 by anion exchange and hydrophobic interaction chromatography Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Sulfurisphaera tokodaii ?
-
 ?
(2R,3S)-3-isopropylmalate + NAD+ the enzyme is enzyme involved in leucine biosynthesis Sulfurisphaera tokodaii ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
IPMDH
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 95 2% of maximal activity at 25°C, maximal activity at 95°C Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant F3S/S232G Sulfurisphaera tokodaii
6
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant R284G Sulfurisphaera tokodaii
6.8
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant F3S Sulfurisphaera tokodaii
6.9
-
 3-isopropylmalate pH 7.5, 70°C, recombinant wild-type enzyme Sulfurisphaera tokodaii
7.5
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant L288P Sulfurisphaera tokodaii
7.8
-
 3-isopropylmalate pH 7.5, 70°C, recombinant mutant F3S/L288P Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sulfurisphaera tokodaii