A335E |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows increased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
D184H |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows increased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
F53L |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows decreased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
H179K |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows decreased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
L134N |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows increased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
additional information |
designing thermostable 3-isopropylmalate dehydrogenase designed by using a phylogenetic tree and ancestral mutant sequences, overview |
Thermus thermophilus |
P324T |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows increased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
P56E |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows increased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
R58L |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows decreased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
S261N |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows decreased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
T16V |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows increased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
V181T |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows increased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |
V61I |
site-directed mutagenesis, exchange of a cofactor binding residue to the residue of the ancestral mutant, the mutant shows decreased thermal stability compared to the wild-type enzyme |
Thermus thermophilus |