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Literature summary for 1.1.1.85 extracted from

  • Matsunami, H.; Kawaguchi, H.; Inagaki, K.; Eguchi, T.; Kakinuma, K.; Tanaka, H.
    Overproduction and substrate specificity of 3-isopropylmalate dehydrogenase from Thiobacillus ferrooxidans (1998), Biosci. Biotechnol. Biochem., 62, 372-373.
    View publication on PubMed
Search for more literature for 1.1.1.85

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 3-ethylmalate
-
 Acidithiobacillus ferrooxidans
0.015
-
 (2R,3S)-3-isopropylmalate
-
 Acidithiobacillus ferrooxidans
0.05
-
 3-methylmalate
-
 Acidithiobacillus ferrooxidans
0.074
-
 3-tert-butylmalate
-
 Acidithiobacillus ferrooxidans
0.11
-
 3-isoamylmalate
-
 Acidithiobacillus ferrooxidans
7.7
-
 malate
-
 Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+ Acidithiobacillus ferrooxidans key enzyme in leucine biosynthesis 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression in Escherichia coli Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
(2R,3S)-3-isopropylmalate + NAD+ key enzyme in leucine biosynthesis Acidithiobacillus ferrooxidans 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
(2R,3S)-3-isopropylmalate + NAD+
-
 Acidithiobacillus ferrooxidans (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 r
3-ethylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-ethylpyruvate + NADH + CO2
-
 ?
3-isoamylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-isoamylpyruvate + NADH + CO2
-
 ?
3-methylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-methylpyruvate + NADH + CO2
-
 ?
3-tert-butylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-tert-butylpyruvate + NADH + CO2
-
 ?
malate + NAD+
-
 Acidithiobacillus ferrooxidans pyruvate + NADH + H+ + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
 3-tert-butylmalate
-
 Acidithiobacillus ferrooxidans
5
-
 malate
-
 Acidithiobacillus ferrooxidans
20
-
 3-isoamylmalate
-
 Acidithiobacillus ferrooxidans
28
-
 (2R,3S)-3-isopropylmalate
-
 Acidithiobacillus ferrooxidans
28
-
 3-methylmalate
-
 Acidithiobacillus ferrooxidans
38
-
 3-ethylmalate
-
 Acidithiobacillus ferrooxidans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Acidithiobacillus ferrooxidans