Crystallization (Comment) | Organism |
---|---|
mutant enzymes A172V, A172G and A172F. As in the case of A172L enzyme, the A172F mutant can not be crystallized by the salting-out technique with ammonium sulfate. The crystal is obtained at pH 4.8 using polyethylene glycol 4000 as a precipitant. Crystals of mutant enzyme A172E are obtained from a drop equilibrated with reservoir solution consisting of 0.8 M ammonium sulfate pH 6.0 at either 15°C or 20°C K. Two types of crystals: one hexagonal bipyramidal and the other is tetragonal | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
A172D | melting temperature is reduced by 2.7°C compared to wild-type enzyme | Thermus thermophilus |
A172F | mutation causes rearrangement in domain structure, which leads to a higher thermostability compared to wild-type enzyme | Thermus thermophilus |
A172F | melting temperature is increased by 1.8°C compared to wild-type enzyme | Thermus thermophilus |
A172G | melting temperature is reduced by 0.3°C compared to wild-type enzyme, crystal structure is similar to that of the wild-type enzyme | Thermus thermophilus |
A172I | melting temperature is increased by 2°C compared to wild-type enzyme | Thermus thermophilus |
A172L | mutation causes rearrangement in domain structure, which leads to a higher thermostability compared to wild-type enzyme | Thermus thermophilus |
A172L | melting temperature is increased by 3°C compared to wild-type enzyme | Thermus thermophilus |
A172V | melting temperature is increased by 1°C compared to wild-type enzyme, crystal structure is similar to that of the wild-type enzyme | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermus thermophilus |
61 | - |
midpoint of thermal unfolding curve, mutant enzyme A172D | Thermus thermophilus |
64 | - |
midpoint of thermal unfolding curve, mutant enzyme A172G | Thermus thermophilus |
65 | - |
midpoint of thermal unfolding curve, mutant enzyme A172V | Thermus thermophilus |
66 | - |
midpoint of thermal unfolding curve, mutant enzyme A172I and A172E | Thermus thermophilus |
67 | - |
midpoint of thermal unfolding curve, mutant enzyme A172L and A172F | Thermus thermophilus |
87 | - |
clear correlation was observed between the hyrophobicity and the thermostability of the enzyme | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |